%0 
%0 Journal Article
%A Herzog, Peter; Winkler, Ilka; Wolking, Dorothee; Kämpfer, Peter & Lipski, André
%D 2008
%T Chryseobacterium ureilyticum sp. nov., Chryseobacterium gambrini sp. nov., Chryseobacterium pallidum sp. nov. and Chryseobacterium molle sp. nov., isolated from beer-bottling plants
%E 
%B International journal of systematic and evolutionary microbiology
%C 
%I 
%V 58
%6
%N 
%P 26-33
%& 
%Y 
%S 
%7
%8 
%9
%? 
%! 
%Z 
%@ 14665026
%(
%)
%*
%L
%M
%1 
%2 
%3 article
%4 
%# 
%$ 
%F herzog_chryseobacterium_2008
%K 16S Bacterial_Typing_Techniques Beer Biofilms Chryseobacterium DNA Fatty_Acids Genotype IFZ Industrial_Microbiology Molecular_Sequence_Data Nucleic_Acid_Hybridization Phenotype Phylogeny RNA Ribosomal Sequence_Analysis Species_Specificity Steel 
%X Four Gram-negative, rod-shaped, non-spore-forming and non-motile bacterial strains were isolated from surfaces and biofilms associated with beer-bottling plants. Based on their 16S rRNA gene sequences these isolates were allocated to the genus Chryseobacterium. The sequence similarities of the isolates to the next most closely related type strains of this genus ranged from 96.4 to 98.3\%. The presence of menaquinone MK-6 and predominant fatty acids 15:0 iso, 17:1 iso cis9, 15:0 iso 2-OH and 17:0 iso 3-OH supported the affiliation of these strains to the genus. The results of DNA-DNA hybridization, biochemical tests and chemotaxonomic properties allowed genotypic and phenotypic differentiation of the strains from the next most closely related Chryseobacterium species with validly published names. Therefore, the isolates represent four novel species for which the names Chryseobacterium ureilyticum (type strain F-Fue-04IIIaaaa(T)=DSM 18017(T)=CCUG 52546(T)), Chryseobacterium gambrini (type strain 5-1St1a(T)=DSM 18014(T)=CCUG 52549(T)), Chryseobacterium pallidum (type strain 26-3St2b(T)=DSM 18015(T)=CCUG 52548(T)) and Chryseobacterium molle (type strain DW3(T)=DSM 18016(T)=CCUG 52547(T)) are proposed.
%Z PMID: 18175677
%U 
%+
%^

%0 
%0 Journal Article
%A Kämpfer, Peter; Falsen, Enevold & Busse, Hans-Jürgen
%D 2008
%T Reclassification of Pseudomonas mephitica Claydon and Hammer 1939 as a later heterotypic synonym of Janthinobacterium lividum (Eisenberg 1891) De Ley et al. 1978
%E 
%B International journal of systematic and evolutionary microbiology
%C 
%I 
%V 58
%6
%N 
%P 136-8
%& 
%Y 
%S 
%7
%8 
%9
%? 
%! 
%Z 
%@ 14665026
%(
%)
%*
%L
%M
%1 
%2 
%3 article
%4 
%# 
%$ 
%F kmpfer_reclassification_2008
%K 16S Bacterial_Typing_Techniques DNA Fatty_Acids Genes IFZ Molecular_Sequence_Data Oxalobacteraceae Phenotype Phylogeny Pseudomonas RNA Ribosomal Sequence_Analysis rRNA 
%X Pseudomonas mephitica CCUG 2513(T) has been reinvestigated to clarify its taxonomic position. 16S rRNA gene sequence comparisons demonstrated that this strain clusters phylogenetically closely with Janthinobacterium lividum (99.8\% sequence similarity to the type strain). Investigation of fatty acid patterns, polar lipid profiles, polyamine patterns and quinone systems supported this delineation. Substrate utilization profiles and biochemical characteristics displayed no differences from the type strain of J. lividum, CCUG 2344(T). Therefore, the reclassification of Pseudomonas mephitica as a later heterotypic synonym of Janthinobacterium lividum is proposed, based upon the estimated phylogenetic position derived from 16S rRNA gene sequence data and chemotaxonomic and biochemical data.
%Z PMID: 18175698
%U 
%+
%^

%0 
%0 Journal Article
%A Kämpfer, Peter; Scholz, Holger C; Huber, Birgit; Falsen, Enevold & Busse, Hans-Jürgen
%D 2007
%T Ochrobactrum haematophilum sp. nov. and Ochrobactrum pseudogrignonense sp. nov., isolated from human clinical specimens
%E 
%B International journal of systematic and evolutionary microbiology
%C 
%I 
%V 57
%6
%N 
%P 2513-8
%& 
%Y 
%S 
%7
%8 November
%9
%? 
%! 
%Z 
%@ 14665026
%(
%)
%*
%L
%M
%1 
%2 
%3 article
%4 
%# 
%$ 
%F kmpfer_ochrobactrum_2007
%K 16S Bacterial Bacterial_Typing_Techniques DNA Fatty_Acids Genes Genotype Gram-Negative_Bacterial_Infections Humans IFZ Molecular_Sequence_Data Nucleic_Acid_Hybridization Ochrobactrum Phenotype Phylogeny RNA Ribosomal Sequence_Analysis rRNA 
%X Three Gram-negative, rod-shaped, non-spore-forming bacteria were isolated from clinical specimens between 1992 and 2000. On the basis of 16S rRNA gene sequence similarities, these strains (CCUG 30717T, CCUG 43892 and CCUG 38531T) were shown to belong to the Alphaproteobacteria, most closely related to Ochrobactrum grignonense (99.0 and 98.2\% similarity to the type strain). Chemotaxonomic data (major ubiquinone Q-10; major polyamines spermidine, sym-homospermidine and putrescine; major polar lipids phosphatidylethanolamine, phosphatidylmonomethylethanolamine, phosphatidylglycerol and phosphatidylcholine; major fatty acids C18:1omega7c and C19:0 cyclo omega8c) supported the affiliation of the isolates to the genus Ochrobactrum. The results of DNA-DNA hybridization and physiological and biochemical tests allowed genotypic and phenotypic differentiation of the isolates from described Ochrobactrum species. Isolates CCUG 30717T and CCUG 43892 were closely related on the basis of DNA-DNA reassociation experiments and therefore represent one novel species, for which the name Ochrobactrum pseudogrignonense sp. nov. is proposed, with the type strain CCUG 30717T (=CIP 109451T). Isolate CCUG 38531T was different from these strains and also from other Ochrobactrum species. For this strain, the name Ochrobactrum haematophilum sp. nov. is proposed, with the type strain CCUG 38531T (=CIP 109452T).
%Z PMID: 17978211
%U 
%+
%^

%0 
%0 Journal Article
%A Scholz, Holger C; Hubalek, Zdenek; Sedlácek, Ivo; Vergnaud, Gilles; Tomaso, Herbert; Dahouk, Sascha Al; Melzer, Falk; Kämpfer, Peter; Neubauer, Heinrich; Cloeckaert, Axel; Maquart, Marianne; Zygmunt, Michel S; Whatmore, Adrian M; Falsen, Enevold; Bahn, Peter; Göllner, Cornelia; Pfeffer, Martin; Huber, Birgit; Busse, Hans-Jürgen & Nöckler, Karsten
%D 2008
%T Brucella microti sp. nov., isolated from the common vole Microtus arvalis
%E 
%B International journal of systematic and evolutionary microbiology
%C 
%I 
%V 58
%6
%N 
%P 375-82
%& 
%Y 
%S 
%7
%8 February
%9
%? 
%! 
%Z 
%@ 14665026
%(
%)
%*
%L
%M
%1 
%2 
%3 article
%4 
%# 
%$ 
%F scholz_brucella_2008
%K 16S Animals Arvicolinae Bacterial Bacterial_Outer_Membrane_Proteins Bacterial_Typing_Techniques Brucella Brucellosis DNA Genes Genotype IFZ Minisatellite_Repeats Molecular_Sequence_Data Nucleic_Acid_Hybridization Phenotype Phylogeny RNA Rec_A_Recombinases Ribosomal Rodent_Diseases Sequence_Analysis Species_Specificity rRNA 
%X Two Gram-negative, non-motile, non-spore-forming, coccoid bacteria (strains CCM 4915(T) and CCM 4916), isolated from clinical specimens of the common vole Microtus arvalis during an epizootic in the Czech Republic in 2001, were subjected to a polyphasic taxonomic study. On the basis of 16S rRNA (rrs) and recA gene sequence similarities, both isolates were allocated to the genus Brucella. Affiliation to Brucella was confirmed by DNA-DNA hybridization studies. Both strains reacted equally with Brucella M-monospecific antiserum and were lysed by the bacteriophages Tb, Wb, F1 and F25. Biochemical profiling revealed a high degree of enzyme activity and metabolic capabilities not observed in other Brucella species. The omp2a and omp2b genes of isolates CCM 4915(T) and CCM 4916 were indistinguishable. Whereas omp2a was identical to omp2a of brucellae from certain pinniped marine mammals, omp2b clustered with omp2b of terrestrial brucellae. Analysis of the bp26 gene downstream region identified strains CCM 4915(T) and CCM 4916 as Brucella of terrestrial origin. Both strains harboured five to six copies of the insertion element IS711, displaying a unique banding pattern as determined by Southern blotting. In comparative multilocus VNTR (variable-number tandem-repeat) analysis (MLVA) with 296 different genotypes, the two isolates grouped together, but formed a separate cluster within the genus Brucella. Multilocus sequence typing (MLST) analysis using nine different loci also placed the two isolates separately from other brucellae. In the IS711-based AMOS PCR, a 1900 bp fragment was generated with the Brucella ovis-specific primers, revealing that the insertion element had integrated between a putative membrane protein and cboL, encoding a methyltransferase, an integration site not observed in other brucellae. Isolates CCM 4915(T) and CCM 4916 could be clearly distinguished from all known Brucella species and their biovars by means of both their phenotypic and molecular properties, and therefore represent a novel species within the genus Brucella, for which the name Brucella microti sp. nov. with the type strain CCM 4915(T) (=BCCN 07-01(T)=CAPM 6434(T)) is proposed.
%Z PMID: 18218934
%U 
%+
%^

%0 
%0 Journal Article
%A Vaneechoutte, Mario; Kämpfer, Peter; Baere, Thierry De; Avesani, Véronique; Janssens, Michèle & Wauters, Georges
%D 2007
%T Chryseobacterium hominis sp. nov., to accommodate clinical isolates biochemically similar to CDC groups II-h and II-c
%E 
%B International journal of systematic and evolutionary microbiology
%C 
%I 
%V 57
%6
%N 
%P 2623-8
%& 
%Y 
%S 
%7
%8 November
%9
%? 
%! 
%Z 
%@ 14665026
%(
%)
%*
%L
%M
%1 
%2 
%3 article
%4 
%# 
%$ 
%F vaneechoutte_chryseobacterium_2007
%K 16S Bacterial Bacterial_Typing_Techniques Centers_for_Disease_Control_and_Prevention_(U.S.) Chryseobacterium DNA Flavobacteriaceae_Infections Genes Humans IFZ Molecular_Sequence_Data Nucleic_Acid_Hybridization Phenotype Phylogeny Polymerase_Chain_Reaction RNA Ribosomal Sequence_Analysis Transfer United_States rRNA 
%X A collection of eight clinical strains from Belgian hospitals and three clinical strains of the CCUG collection were characterized biochemically as being similar to CDC groups II-h and II-c; the latter differs from group II-h only by positivity for sucrose acidification. These 11 strains were found to cluster according to 16S rRNA gene sequence similarity at a level of {\textgreater}or=99.5\%, and on the basis of their tDNA-PCR profile. Based on 16S rRNA gene sequence analysis, this collection of strains was related most closely to Chryseobacterium hispanicum (97.2\%), but they differed from the type strain of this species by the following phenotypic characteristics: growth at 37 degrees C, negativity for xylose acidification, positivity for acetate assimilation-alkalinization on Simmons' agar base and absence of flexirubin pigments, and by their tDNA-PCR profile. Strain NF802T showed only 57.8\% DNA-DNA relatedness to the type strain of C. hispanicum. Fatty acid composition did not enable differentiation from C. hispanicum. The DNA G+C content of strain NF802T is 36.5 mol\%. The name Chryseobacterium hominis sp. nov. is proposed for this taxon, with type strain NF802T (=CCUG 52711T=CIP 109415T).
%Z PMID: 17978230
%U 
%+
%^