QuickSearch:   Number of matching entries: 0.

Search Settings

    AuthorTitleYearJournal/ProceedingsReftypeDOI/URL
    Feller, M.B., Wellis, D.P., Stellwagen, D., Werblin, F.S. & Shatz, C.J. Requirement for cholinergic synaptic transmission in the propagation of spontaneous retinal waves.

    [Abstract] [BibTeX]

    		 1996 Science
    Vol. 272(5265), pp. 1182-1187     		 article  
    Abstract: Highly correlated neural activity in the form of spontaneous waves of action potentials is present in the developing retina weeks before vision. Optical imaging revealed that these waves consist of spatially restricted domains of activity that form a mosaic pattern over the entire retinal ganglion cell layer. Whole-cell recordings indicate that wave generation requires synaptic activation of neuronal nicotinic acetylcholine receptors on ganglion cells. The only cholinergic cells in these immature retinas are a uniformly distributed bistratified population of amacrine cells, as assessed by antibodies to choline acetyltransferase. The results indicate that the major source of synaptic input to retinal ganglion cells is a system of cholinergic amacrine cells, whose activity is required for wave propagation in the developing retina.
    BibTeX: 
    @article{Feller1996,
  author = {M. B. Feller and D. P. Wellis and D. Stellwagen and F. S. Werblin and C. J. Shatz},
  title = {Requirement for cholinergic synaptic transmission in the propagation of spontaneous retinal waves.},
  journal = {Science},
  year = {1996},
  volume = {272},
  number = {5265},
  pages = {1182--1187}
}
    Feller, M.B., Wellis, D.P., Stellwagen, D., Werblin, F.S. & Shatz, C.J. Requirement for Cholinergic Synaptic Transmission in the Propagation of Spontaneous Retinal Waves

    [BibTeX]

    		 1996 Science
    Vol. 272, pp. 1182-1187     		 article URL 
    BibTeX: 
    @article{feller:science96,
  author = {Marla B. Feller and David P. Wellis and David Stellwagen and Frank S. Werblin and Carla J. Shatz},
  title = {Requirement for Cholinergic Synaptic Transmission in the Propagation of Spontaneous Retinal Waves},
  journal = {Science},
  year = {1996},
  volume = {272},
  pages = {1182--1187},
  url = {http://links.jstor.org/sici?sici=0036-807581996052493A272A5265C1182ARFCSTIE2.0.COB2-U}
}
    Feller, M.B., Wellis, D.P., Stellwagen, D., Werblin, F.S. & Shatz, C.J. Requirement for Cholinergic Synaptic Transmission in the Propagation of Spontaneous Retinal Waves

    [BibTeX]

    		 1996 Science
    Vol. 272, pp. 1182-1187     		 article URL 
    BibTeX: 
    @article{feller:science96,
  author = {Marla B. Feller and David P. Wellis and David Stellwagen and Frank S. Werblin and Carla J. Shatz},
  title = {Requirement for Cholinergic Synaptic Transmission in the Propagation of Spontaneous Retinal Waves},
  journal = {Science},
  year = {1996},
  volume = {272},
  pages = {1182--1187},
  url = {http://links.jstor.org/sici?sici=0036-807581996052493A272A5265C1182ARFCSTIE2.0.COB2-U}
}
    Mohanty, U. & Stellwagen, N.C. Free solution mobility of oligomeric DNA

    [Abstract] [BibTeX]

    		 1999 Biopolymers
    Vol. 49(3), pp. 209-214     		 article  
    Abstract: The free solution electrophoretic mobility of a charged oligomer in an ionic solvent that approximately takes into account relaxation field effects, screening of the velocity field and the hydrodynamic interactions resulting from motions of the charges due to an electric field is described. For double-stranded DNA, the free solution electrophoretic mobility under ionic strengths determined by the buffer and pH conditions relevant to capillary electrophoresis increases with increasing molecular weight up to few hundred base pairs. (C) 1999 John Wiley & Sons, Inc.
    BibTeX: 
    @article{mohanty99a,
  author = {U. Mohanty and N. C. Stellwagen},
  title = {Free solution mobility of oligomeric DNA},
  journal = {Biopolymers},
  year = {1999},
  volume = {49},
  number = {3},
  pages = {209--214}
}
    Stellwagen, D. & Shatz, C.J. An Instructive Role for Retinal Waves in the Development of Retinogeniculate Connectivity

    [BibTeX]

    		 2002 Neuron
    Vol. 33(3), pp. 357-367     		 article URL 
    BibTeX: 
    @article{stellwagen:neuron02,
  author = {David Stellwagen and Carla J. Shatz},
  title = {An Instructive Role for Retinal Waves in the Development of Retinogeniculate Connectivity},
  journal = {Neuron},
  year = {2002},
  volume = {33},
  number = {3},
  pages = {357--367},
  url = {http://www.neuron.org/cgi/content/abstract/33/3/357}
}
    Stellwagen, D. & Shatz, C.J. An Instructive Role for Retinal Waves in the Development of Retinogeniculate Connectivity

    [BibTeX]

    		 2002 Neuron
    Vol. 33(3), pp. 357-367     		 article URL 
    BibTeX: 
    @article{stellwagen:neuron02,
  author = {David Stellwagen and Carla J. Shatz},
  title = {An Instructive Role for Retinal Waves in the Development of Retinogeniculate Connectivity},
  journal = {Neuron},
  year = {2002},
  volume = {33},
  number = {3},
  pages = {357--367},
  url = {http://www.neuron.org/cgi/content/abstract/33/3/357}
}
    Stellwagen, E., Lu, Y.J. & Stellwagen, N.C. Unified description of electrophoresis and diffusion for DNA and other polyions

    [Abstract] [BibTeX]

    		 2003 Biochemistry
    Vol. 42(40), pp. 11745-11750     		 article  
    Abstract: The electrophoretic mobilities and diffusion coefficients of single- and double-stranded DNA molecules up to 50 000 bases or base pairs in size have been analyzed, using mobilities and diffusion coefficients either measured by capillary electrophoresis or taken from the literature. The Einstein equation suggests that the electrophoretic mobilities (mu) and diffusion coefficients (D) should be related by the xpression mu/D = Q/k(B)T, where Q is the charge of the polyion (Q = ze(o), where z is the number of charged residues and e(o) is the fundamental electronic charge), k(B) is Boltzmann's constant, and T is the absolute temperature. If this equation were true, the ratio mu/zD should be a constant equal to e(o)/k(B)T (39.6 V-1) at 20 degreesC. However, the ratio mu/zD decreases with an increase in molecular weight for both single-and double-stranded DNAs. The mobilities and diffusion coefficients are better described by the modified Einstein equation mu/(ND)-D-m = e(o)/k(B)T, where N is the number of repeat units (bases or base pairs) in the DNA and m is a constant equal to the power law dependence of the diffusion coefficients on molecular weight. The average value of the ratio mu/(ND)-D-m is 40 +/- 4 V-1 for 36 single- and double-stranded DNA molecules of different sizes, close to the theoretically expected value. The generality of the modified Einstein equation is demonstrated by analyzing literature values for sodium polystyrenesulfonate (PSS). The average value of the ratio mu/(ND)-D-m is 35 +/- 6 V-1 for 14 PSS samples containing up to 855 monomers.
    BibTeX: 
    @article{stellwagen03a,
  author = {E. Stellwagen and Y. J. Lu and N. C. Stellwagen},
  title = {Unified description of electrophoresis and diffusion for DNA and other polyions},
  journal = {Biochemistry},
  year = {2003},
  volume = {42},
  number = {40},
  pages = {11745--11750}
}
    Stellwagen, E. & Stellwagen, N.C. Determining the electrophoretic mobility and translational diffusion coefficients of DNA molecules in free solution

    [Abstract] [BibTeX]

    		 2002 Electrophoresis
    Vol. 23(16), pp. 2794-2803     		 article  
    Abstract: The free solution mobility of DNA molecules of different molecular weights, the sequence dependence of the mobility, and the diffusion coefficients of small single- and double-stranded DNA (ss- and dsDNA) molecules can be measured accurately by capillary zone electrophoresis, using coated capillaries to minimize the electro-osmotic flow (EOF) of the solvent. Very small differences in mobility between various analytes can be quantified if a mobility marker is used to correct for small differences in EOF between successive experiments. Using mobility markers, the molecular weight at which the free solution mobility of dsDNA becomes independent of molecular weight is found to be similar to 170 bp in 40 mm Tris-acetate-EDTA buffer. A DNA fragment containing 170 bp has a contour length of similar to 58 nm, close to the persistence length of DNA under these buffer conditions. Hence, the approach of the free solution mobility of DNA to a plateau value may be associated with the transition from a rod-like to a coil-like conformation in solution. Markers have also been used to determine that the free solution mobilities of ss- and dsDNA oligomers are sequence-dependent. Double-stranded 20-bp oligomers; containing runs of three or more adenine residues in a row (A-tracts) migrate somewhat more slowly than 20-mers without A-tracts, suggesting that somewhat larger numbers of counterions are condensed in the ion atmospheres of A-tract DNAs, decreasing their net effective charge. Single-stranded 20-mers with symmetric sequences migrate similar to 1 % faster than their double-stranded counterparts, and faster than single-stranded 20-mers containing A(5)- or T-5-tracts. Interestingly, the average mobility of two complementary single-stranded 20-mers is equal to the mobility of the double-stranded oligomer formed upon annealing. Finally, the stopped migration method has been used to measure the diffusion coefficients of single- and double- stranded oligomers. The diffusion coefficients of ssDNA oligomers containing 20 nucleotides are similar to 50% larger than those of double-stranded DNA oligomers of the same size, reflecting the greater flexibility of ssDNA molecules. The methods used to carry out these experiments are also described in detail.
    BibTeX: 
    @article{stellwagen02a,
  author = {E. Stellwagen and N. C. Stellwagen},
  title = {Determining the electrophoretic mobility and translational diffusion coefficients of DNA molecules in free solution},
  journal = {Electrophoresis},
  year = {2002},
  volume = {23},
  number = {16},
  pages = {2794--2803}
}
    Stellwagen, N.C., Gelfi, C. & Righetti, P.G. The free solution mobility of DNA

    [Abstract] [BibTeX]

    		 1997 Biopolymers
    Vol. 42(6), pp. 687-703     		 article  
    Abstract: The free solution mobility of DNP, has been measured by capillary electrophoresis in the two buffers most commonly used for DNA gel electrophoresis,;Tris-borate-EDTA (TEE) and Tris-acetate-EDTA (TAE). The capillaries were coated with polymers of either of two novel acrylamide monomers, N-acryloylaminoethoxethanol or N-acryloylaminopropanol, both of which are stable at basic pH and effectively eliminate the electroendosmotic,nobility due to the capillary walls. The free solution mobility of DNA in TAE buffer was found to be (3.75 +/- 0.04) x 10(-4) cm(2) V-1 s(-1) at 25 degrees C. independent of DNA concentration, sample size, electric field strength, and capillary coating, and in good agreement with other values in the literature. The free solution mobility was independent of DNA molecular weight from similar to 400 base pairs to 48.5 kilobase pairs, but decreased monotonically with decreasing molecular weight for smaller fragments. Surprisingly, the free solution mobility of DNA in TEE buffer was found to be (4.5 +/- 0.1) x 10(-4) cm(2) V-1 s(-1), about 20% larger than observed in TAE buffer, presumably because of the formation of nonspecific borate-deoxyribose complexes. (C) 1997 John Wiley & Sons, Inc.
    BibTeX: 
    @article{stellwagen97a,
  author = {N. C. Stellwagen and C. Gelfi and P. G. Righetti},
  title = {The free solution mobility of DNA},
  journal = {Biopolymers},
  year = {1997},
  volume = {42},
  number = {6},
  pages = {687--703}
}
    Stellwagen, N.C., Magnusdottir, S., Gelfi, C. & Righetti, P.G. Measuring the translational diffusion coefficients of small DNA molecules by capillary electrophoresis

    [Abstract] [BibTeX]

    		 2001 Biopolymers
    Vol. 58(4), pp. 390-397     		 article  
    Abstract: The apparent translational diffusion coefficients of four 20 base pair (bp) DNA oligonucleotides with different sequences have been measured by capillary electrophoresis. using the stopped migration method The diffusion coefficients of the four oligomers were equal within experimental error, and averaged (120 +/- 10) x 10(-8) cm(2) s(-1) in 40 mM Tris-acetate-EDTA buffer at 25 degreesC. Since this value is nearly identical to the translational diffusion coefficient determined for a different 20-bp oligomer using other methods, the stopped migration method can accurately measure the diffusion coefficients of small DNA oligomers. The apparent diffusion coefficient of a 118-bp DNA restriction fragment was also measured by the stopped migration method. However. the observed value was similar to 25% larger than expected from other measurements, possibly because the diffusion coefficients of larger DNA molecules are somewhat dependent on the ionic strength of the solution. (C) 2001 John Wiley & Sons, Inc. Biopolymers 58: 390-397, 2001.
    BibTeX: 
    @article{stellwagen01a,
  author = {N. C. Stellwagen and S. Magnusdottir and C. Gelfi and P. G. Righetti},
  title = {Measuring the translational diffusion coefficients of small DNA molecules by capillary electrophoresis},
  journal = {Biopolymers},
  year = {2001},
  volume = {58},
  number = {4},
  pages = {390--397}
}

    Created by JabRef on 06/09/2008.