Pollution of aquatic ecosystems can be ascertained through the organisms residing in it. Biochemical changes in fish reflect the type of degradation occurring in living systems under the influence of any toxicant. Naphthalene is easily absorbed and distributed inside the body and is metabolized mainly by liver. Anabas testudineus was used as toxicity test organism to analyze various forms of damage. Basic water quality indices were checked before designing the bioassay test. Lethal concentration Of Naphthalene which killed 50% of Test animals (LC50) was determined after exposing the fish to varying concentrations for a period of 96 hours. The test specimen selected had average weight of 2-5gm and was procured from Central Institute of Freshwater Aquaculture (CIFA). After exposure to Naphthalene, the test organism showed major changes in the glycogen content, acetylcholine esterase, protein and adenosine triphosphate with increase in toxicant concentration. Constant decrease in the biochemical parameters indicates stress condition of Anabas testudineus.
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