Pesticides are chemical compounds used to control insects and weeds to enhance food and crops productivity. The present study was aimed to evaluate the effects of pesticides on health status of dealers and farmers in district Dir Lower, Khyber Pakhtunkhwa, Pakistan. Blood samples were collected from different groups viz: farmers, dealers (exposed to pesticides), and control (not exposed to pesticides) and the history was recorded on a questioner. For Blood Profiling and Serum Biomarkers analysis, Sysmex Kx-21 (Japan) and Shiamadzu Double Beam Spectrophotometer 1700 Pharma (Japan) were used. Upon pesticides exposure the blood cholesterol, Triglyceride, Serum glutamate pyruvate transaminase, Uric Acid, Total Lipid, Low density lipoprotein, High density lipoprotein, Mean Corpuscular Hemoglobin Concentration, Platelets and White Blood Cells count were increased in dealers and farmers as compared to control group. While, Hemoglobin, Red Blood Cells, hematocrit, Mean Corpuscular Volume, Mean Corpuscular Hemoglobin, Lymphocytes and Neutrophils counts decreased in farmers and dealers as compared to control. The present findings suggest that pesticides exposure adversely affect dealers and farmers health status by significantly altering their liver and kidney functions as well as blood biochemistry and hematology.