A major part of the human di et al over the world consists of cereals and legumes. Legumes are however, considered as the major source of protein and dietary amino acid for man and farm animals. The detailed evolutionary relations and cluster analysis in this group of plants may help in further manipulations and modifications of plants leading to enhanced nutritional supplementation for consumption. To estimatethe picture of genetic diversity in lentil (Lens culinaris)the proteomics assay was carried out. A total of 60 cultivars were collected from 60 lentil growing areas of Malakand Division Khyber Pakhtunkhwa, Pakistan. The obtained lines were biochemically evaluated for the purpose to check the genetic heterogeneity. Based on proteomics homology dendrogram and cluster plottingsorted 60 cultivars into 4 clusters i.e. Cluster-1 (sorted forty cultivars), cluster-2 (grouped single cultivar), cluster-3 (had seventeen genotypes) and cluster-4 (grouped two cultivars).Thirteen loci (bands) were spotted in the collected germplasm of lentils through SDS-PAGE. Intra species locus contribution toward genetic diversity (LCTGD) was 84.61. Out of thirteen loci, locus-1, 2, 3,4,5, 6,7,8, 9, 10 and 11 showed polymorphic.The results showed that SDS-PAGE displayed a low level Genetic polymorphism in our accessions but higher than in other legumes, and it can be increased by the addition of more extensively sourced germplasm.