Menthea piperita from Lamiaceae family is one of medicinal plants which is widely used in pharmaceutical, food and hygienic industries. Organic and bio-fertilizers consumption in medicinal plants production and sustainable agriculture is important for achieving high quality of yield, environment protect and society health. Therefore, a experiment was conducted to investigate the effect of organic fertilizers on Menthea piperita in the research greenhouse of Islamic Azad University of Karaj, Iran. It was done in randomized complete block design with four replications in the autumn and winter of 2014. Treatments were imposed based on pots volume with vermicompost (5, 15 and 30%), peat moss (70, 85 and 100%), biochar (use or non-use) and mycorrhiza (use or non-use) combination. The analysis of variance indicated that treatments were different for stem fresh weight, leaf fresh weight, weight of total dry matter and shed leaves weight (α≤ 0.05), and for root dry weight (α≤ 0.01). Different fertilizer treatments had no significant effect on stem, leaf and soot dry weight. The highest leaf fresh yield belonged to 15% of vermicompost, 85% of peat moss, non-use of mycorrhiza and biochar treatment with 39.15 gram per pot. It seems that vermicompost with chemical fertilizers could be increased photosynthesis, dry matter amount, yield and growth of the plant due to supplying its nutrients in absorbable form.