%0 Journal Article %1 Jungmann2014a %A Jungmann, Ralf %A Avendaño, Maier S. %A Woehrstein, Johannes B. %A Dai, Mingjie %A Shih, William M. %A Yin, Peng %D 2014 %I Nature Publishing Group %J Nature Methods %K imaging microscopy paint smlm superresolution %N 3 %P 313--318 %R 10.1038/nmeth.2835 %T Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT %U http://www.nature.com/articles/nmeth.2835 %V 11 %X Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures. We also report a multiplexing approach (Exchange-PAINT) that allows sequential imaging of multiple targets using only a single dye and a single laser source. We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional (2D) imaging and three-color 3D imaging of proteins in fixed cells. © 2014 Nature America, Inc. %@ 1548-7105 (Electronic)$\backslash$r1548-7091 (Linking)

@article{Jungmann2014a, abstract = {Super-resolution fluorescence microscopy is a powerful tool for biological research, but obtaining multiplexed images for a large number of distinct target species remains challenging. Here we use the transient binding of short fluorescently labeled oligonucleotides (DNA-PAINT, a variation of point accumulation for imaging in nanoscale topography) for simple and easy-to-implement multiplexed super-resolution imaging that achieves sub-10-nm spatial resolution in vitro on synthetic DNA structures. We also report a multiplexing approach (Exchange-PAINT) that allows sequential imaging of multiple targets using only a single dye and a single laser source. We experimentally demonstrate ten-color super-resolution imaging in vitro on synthetic DNA structures as well as four-color two-dimensional (2D) imaging and three-color 3D imaging of proteins in fixed cells. {\textcopyright} 2014 Nature America, Inc.}, added-at = {2020-04-06T13:11:22.000+0200}, archiveprefix = {arXiv}, arxivid = {NIHMS150003}, author = {Jungmann, Ralf and Avenda{\~{n}}o, Maier S. and Woehrstein, Johannes B. and Dai, Mingjie and Shih, William M. and Yin, Peng}, biburl = {https://www.bibsonomy.org/bibtex/20d7882a242fce47e0628df9124ac3988/kfriedl}, doi = {10.1038/nmeth.2835}, eprint = {NIHMS150003}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Jungmann et al. - 2014 - multiplexed 3d cellular super-resolution imaging with dnA-PAint and exchange-PAint(2).pdf:pdf;:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Jungmann et al. - 2014 - Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT.pdf:pdf}, interhash = {06bba3851a3b851b39531e4c6a5be32a}, intrahash = {0d7882a242fce47e0628df9124ac3988}, isbn = {1548-7105 (Electronic)$\backslash$r1548-7091 (Linking)}, issn = {15487105}, journal = {Nature Methods}, keywords = {imaging microscopy paint smlm superresolution}, month = feb, number = 3, pages = {313--318}, pmid = {24487583}, publisher = {Nature Publishing Group}, timestamp = {2020-04-06T14:05:10.000+0200}, title = {{Multiplexed 3D cellular super-resolution imaging with DNA-PAINT and Exchange-PAINT}}, url = {http://www.nature.com/articles/nmeth.2835}, volume = 11, year = 2014 }