The effect of different storage methods on the mechanical properties of trabecular bone.
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J Biomech 26 (10): 1249--1252 (October 1993)

The effect of different storage methods on the elastic and viscoelastic properties of trabecular bone was examined using non-destructive compression tests to 0.45\% specimen strain. There was a 10\% decrease in stiffness during the first 24 h post mortem. Storage by freezing or in ethanol for 100 d did not change the stiffness, and neither did several thawing, testing and refreezing sequences. The viscoelastic properties were more sensitive to storage and showed significant although small changes during 100 d of storage. The effect of defatting the specimens was a 30\% increase in stiffness and a 50\% decrease in viscoelastic energy dissipation.
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