%0 Journal Article %1 Fisher:2011:Genome-Biol:21205303 %A Fisher, S %A Barry, A %A Abreu, J %A Minie, B %A Nolan, J %A Delorey, T M %A Young, G %A Fennell, T J %A Allen, A %A Ambrogio, L %A Berlin, A M %A Blumenstiel, B %A Cibulskis, K %A Friedrich, D %A Johnson, R %A Juhn, F %A Reilly, B %A Shammas, R %A Stalker, J %A Sykes, S M %A Thompson, J %A Walsh, J %A Zimmer, A %A Zwirko, Z %A Gabriel, S %A Nicol, R %A Nusbaum, C %D 2011 %J Genome Biol %K MUSTREAD broad exome fulltext methods next-generation-sequencing sureselect wes %N 1 %R 10.1186/gb-2011-12-1-r1 %T A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries %U https://www.ncbi.nlm.nih.gov/pubmed/21205303/ %V 12 %X Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

@article{Fisher:2011:Genome-Biol:21205303, abstract = {Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.}, added-at = {2017-10-03T14:07:45.000+0200}, author = {Fisher, S and Barry, A and Abreu, J and Minie, B and Nolan, J and Delorey, T M and Young, G and Fennell, T J and Allen, A and Ambrogio, L and Berlin, A M and Blumenstiel, B and Cibulskis, K and Friedrich, D and Johnson, R and Juhn, F and Reilly, B and Shammas, R and Stalker, J and Sykes, S M and Thompson, J and Walsh, J and Zimmer, A and Zwirko, Z and Gabriel, S and Nicol, R and Nusbaum, C}, biburl = {https://www.bibsonomy.org/bibtex/2101e25516c70e4b7ceedca12c54a0d2f/marcsaric}, description = {A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries. - PubMed - NCBI}, doi = {10.1186/gb-2011-12-1-r1}, interhash = {1e7087079eb391f3dcd41e954d4aa443}, intrahash = {101e25516c70e4b7ceedca12c54a0d2f}, journal = {Genome Biol}, keywords = {MUSTREAD broad exome fulltext methods next-generation-sequencing sureselect wes}, number = 1, pmid = {21205303}, timestamp = {2017-10-03T14:07:45.000+0200}, title = {A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries}, url = {https://www.ncbi.nlm.nih.gov/pubmed/21205303/}, volume = 12, year = 2011 }