Zusammenfassung
In this work, we have studied the effect of Crotalus basiliscus snake
venom on the redox reaction of myoglobin (Mb), and by means of
electrochemical techniques, we have shown that this reaction is
undoubtedly affected following the interaction with the venom. Surface
plasmon resonance, electrophoresis, UV-Vis, and circular dichroism
showed that the interaction involves the attachment of some constituent
of the venom to the protein, although not affecting its first and
secondary structures. Mass spectra support this suggestion by showing
the appearance of signals assigned to the Mb dimer and to a new species
resulting from the interaction between Mb and the venom proteins. In
addition, the mass spectra suggest the aromatic amino acids of
myoglobin, mainly tryptophan and phenylalanine, are more exposed to the
solvent medium upon the exposure to the venom solution. The results
altogether indicate that the harmful effects of the venom of Crotalus
basiliscus snake are likely connected to the blocking of the redox site
of Mb.
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