Abstract
Biological macromolecules evolve and function within intracellular
environments that are crowded with other macromolecules. Crowding
results in surprisingly large quantitative effects on both the rates
and the equilibria of interactions involving macromolecules, but
such interactions are commonly studied outside the cell in uncrowded
buffers. The addition of high concentrations of natural and synthetic
macromolecules to such buffers enables crowding to be mimicked in
vitro, and should be encouraged as a routine variable to study. The
stimulation of protein aggregation by crowding might account for
the existence of molecular chaperones that combat this effect. Positive
results of crowding include enhancing the collapse of polypeptide
chains into functional proteins, the assembly of oligomeric structures
and the efficiency of action of some molecular chaperones and metabolic
pathways.
- 11590012
- 80
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- adenocarcinoma,
- aged,
- analysis,
- and
- animals,
- assurance,
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- carboxylase,
- care,
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- coli,
- combined
- conformal,
- development,
- escherichia
- factors,
- folding,
- health
- humans,
- in
- indium
- intraoperative
- kinetics,
- macromolecular
- male,
- middle
- mobile
- modality
- models,
- molecular
- neoplasms,
- nursing,
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- outcome,
- over,
- palladium,
- particle
- period,
- perioperative
- preoperative
- program
- prostatic
- protein
- proteins,
- quality
- radioimmunodetection,
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