%0 Journal Article %1 citeulike:504095 %A Groenewegen, P. E. %A Breeuwer, P. %A van Helvoort, J. M. %A Langenhoff, A. A. %A de Vries, F. P. %A de Bont, J. A. %C Department of Food Science, Agricultural University, Wageningen, The Netherlands. %D 1992 %J J Gen Microbiol %K hydroxylamine bio-degradation aromatic-nitro %P 1599--1605 %T Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=1527502 %V 138 Pt 8 %X A Comamonas acidovorans strain, designated NBA-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. When grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. In cell extracts with NADPH present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-nitrosobenzoate to 4-hydroxylamino-benzoate. The substrate specificity of the enzyme was narrow and NADPH was 15 times more effective as a cofactor than NADH. The results provide evidence for a novel pathway for aerobic degradation of 4-nitrobenzoate, since neither 4-hydroxybenzoate nor 4-aminobenzoate were involved in the degradative pathway.

@article{citeulike:504095, abstract = {A Comamonas acidovorans strain, designated NBA-10, was isolated on 4-nitrobenzoate as sole carbon and energy source. When grown on 4-nitrobenzoate, it was simultaneously adapted to 4-nitrosobenzoate and 4-hydroxylaminobenzoate but not to 4-hydroxybenzoate or 4-aminobenzoate. In cell extracts with NADPH present, 4-nitrobenzoate was degraded to 4-hydroxylaminobenzoate and 3,4-dihydroxybenzoate. Partial purification of the 4-nitrobenzoate reductase revealed that 4-nitrobenzoate is degraded via 4-nitrosobenzoate to 4-hydroxylamino-benzoate. The substrate specificity of the enzyme was narrow and NADPH was 15 times more effective as a cofactor than NADH. The results provide evidence for a novel pathway for aerobic degradation of 4-nitrobenzoate, since neither 4-hydroxybenzoate nor 4-aminobenzoate were involved in the degradative pathway.}, added-at = {2007-02-02T11:54:15.000+0100}, address = {Department of Food Science, Agricultural University, Wageningen, The Netherlands.}, author = {Groenewegen, P. E. and Breeuwer, P. and van Helvoort, J. M. and Langenhoff, A. A. and de Vries, F. P. and de Bont, J. A.}, biburl = {https://www.bibsonomy.org/bibtex/24328eabcbe5282c7a4f97cc0f782a1ff/robert}, citeulike-article-id = {504095}, interhash = {81ce6f2b0b5910a7b99919e6574bcef5}, intrahash = {4328eabcbe5282c7a4f97cc0f782a1ff}, issn = {0022-1287}, journal = {J Gen Microbiol}, keywords = {hydroxylamine bio-degradation aromatic-nitro}, month = {August}, pages = {1599--1605}, priority = {2}, timestamp = {2007-02-02T11:54:15.000+0100}, title = {Novel degradative pathway of 4-nitrobenzoate in Comamonas acidovorans NBA-10.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=1527502}, volume = {138 Pt 8}, year = 1992 }