Abstract
A plethora of optical techniques is currently available to obtain
non-destructive, contactless, real time information with subcellular
spatial resolution to observe cell processes. Each technique has its own
unique features for imaging and for obtaining certain biological
information. However none of the available techniques can be of
universal use. For a comprehensive investigation of biological specimens
and events, one needs to use a combination of bioimaging methods, often
at the same time. Some modern confocal/multiphoton microscopes provide
simultaneous fluorescence, fluorescence lifetime imaging, and
four-dimensional imaging. Some of them can also easily be adapted for
harmonic generation imaging, and to permit cell manipulation technique.
In this work we present a multimodal optical workstation that extends a
commercially available confocal microscope to include
nonlinear/multiphoton microscopy and optical manipulation/stimulation
tools. The nonlinear microscopy capabilities were added to the
commercial confocal microscope by exploiting all the flexibility offered
by the manufacturer. The various capabilities of this workstation as
applied directly to reproductive biology are discussed. (C) 2016 Wiley
Periodicals, Inc.
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