In the present study, we followed the CLSI procedure M40-A to evaluate three specimen transport systems the new BD CultureSwab MaxV(+), the new Remel BactiSwab, and the Medical Wire & Equipment Transwab for the survival of fastidious and nonfastidious organisms for 0, 6, 24, and 48 h at room temperature. BD CultureSwab MaxV(+) outperformed the other two swabs for the recovery of the three fastidious organisms, Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis for up to 48 h. Indeed, BD CultureSwab MaxV(+) maintained a constant number of viable H. influenzae and N. meningitidis for up to 48 h, and only a 2 log reduction was noted for N. gonorrhoeae, fulfilling the requirements of M40-A guidelines. However, unlike Remel BactiSwab and the Medical Wire & Equipment Transwab, which fulfilled the M40-A requirements for maintaining the viability of Streptococcus pneumoniae, BD CultureSwab MaxV(+) could not maintain the viability of S. pneumoniae reference or clinical strains past 6 h. Excellent overall sensitivity (98\%) (95\% confidence interval, 89.5 to 99.7) was observed when the BD CultureSwab MaxV(+) rectal swabs were compared to the "gold standard" stool cultures. Thus, the BD CultureSwab MaxV(+) rectal swab can be used when investigating gastrointestinal bacterial outbreaks or when health care providers face difficulties in obtaining stool samples, particularly from children.
%0 Journal Article
%1 rishmawi_survival_2007
%A Rishmawi, Nabeel
%A Ghneim, Raed
%A Kattan, Randa
%A Ghneim, Riyad
%A Zoughbi, Madeleine
%A Abu-Diab, Afaf
%A Turkuman, Sultan
%A Dauodi, Rula
%A Shomali, Issa
%A Issa, Abed El-Razeq
%A Siriani, Issa
%A Marzouka, Hiyam
%A Schmid, Irmgard
%A Hindiyeh, Musa Y
%D 2007
%J Journal of Clinical Microbiology
%K Bacteriological Colony Count, Factors Feces, Haemophilus Handling, Humans, Microbial Microbial, Neisseria Rectum, Sensitivity Specificity, Specimen Streptococcus Techniques, Temperature, Time Viability, and gonorrhoeae, influenzae, meningitidis, pneumoniae, swab,
%N 4
%P 1278--1283
%R 10.1128/JCM.02110-06
%T Survival of fastidious and nonfastidious aerobic bacteria in three bacterial transport swab systems
%U http://www.ncbi.nlm.nih.gov/pubmed/17267627
%V 45
%X In the present study, we followed the CLSI procedure M40-A to evaluate three specimen transport systems the new BD CultureSwab MaxV(+), the new Remel BactiSwab, and the Medical Wire & Equipment Transwab for the survival of fastidious and nonfastidious organisms for 0, 6, 24, and 48 h at room temperature. BD CultureSwab MaxV(+) outperformed the other two swabs for the recovery of the three fastidious organisms, Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis for up to 48 h. Indeed, BD CultureSwab MaxV(+) maintained a constant number of viable H. influenzae and N. meningitidis for up to 48 h, and only a 2 log reduction was noted for N. gonorrhoeae, fulfilling the requirements of M40-A guidelines. However, unlike Remel BactiSwab and the Medical Wire & Equipment Transwab, which fulfilled the M40-A requirements for maintaining the viability of Streptococcus pneumoniae, BD CultureSwab MaxV(+) could not maintain the viability of S. pneumoniae reference or clinical strains past 6 h. Excellent overall sensitivity (98\%) (95\% confidence interval, 89.5 to 99.7) was observed when the BD CultureSwab MaxV(+) rectal swabs were compared to the "gold standard" stool cultures. Thus, the BD CultureSwab MaxV(+) rectal swab can be used when investigating gastrointestinal bacterial outbreaks or when health care providers face difficulties in obtaining stool samples, particularly from children.
@article{rishmawi_survival_2007,
abstract = {In the present study, we followed the {CLSI} procedure {M40-A} to evaluate three specimen transport systems [the new {BD} {CultureSwab} {MaxV(+),} the new Remel {BactiSwab,} and the Medical Wire \& Equipment Transwab] for the survival of fastidious and nonfastidious organisms for 0, 6, 24, and 48 h at room temperature. {BD} {CultureSwab} {MaxV(+)} outperformed the other two swabs for the recovery of the three fastidious organisms, Haemophilus influenzae, Neisseria gonorrhoeae, and Neisseria meningitidis for up to 48 h. Indeed, {BD} {CultureSwab} {MaxV(+)} maintained a constant number of viable H. influenzae and N. meningitidis for up to 48 h, and only a 2 log reduction was noted for N. gonorrhoeae, fulfilling the requirements of {M40-A} guidelines. However, unlike Remel {BactiSwab} and the Medical Wire \& Equipment Transwab, which fulfilled the {M40-A} requirements for maintaining the viability of Streptococcus pneumoniae, {BD} {CultureSwab} {MaxV(+)} could not maintain the viability of S. pneumoniae reference or clinical strains past 6 h. Excellent overall sensitivity (98\%) (95\% confidence interval, 89.5 to 99.7) was observed when the {BD} {CultureSwab} {MaxV(+)} rectal swabs were compared to the "gold standard" stool cultures. Thus, the {BD} {CultureSwab} {MaxV(+)} rectal swab can be used when investigating gastrointestinal bacterial outbreaks or when health care providers face difficulties in obtaining stool samples, particularly from children.},
added-at = {2011-03-11T10:05:34.000+0100},
author = {Rishmawi, Nabeel and Ghneim, Raed and Kattan, Randa and Ghneim, Riyad and Zoughbi, Madeleine and {Abu-Diab}, Afaf and Turkuman, Sultan and Dauodi, Rula and Shomali, Issa and Issa, Abed {El-Razeq} and Siriani, Issa and Marzouka, Hiyam and Schmid, Irmgard and Hindiyeh, Musa Y},
biburl = {https://www.bibsonomy.org/bibtex/22d8d00d9f4f0c5e6937976e063837b89/jelias},
doi = {10.1128/JCM.02110-06},
interhash = {dd9af424fc819921ade7a0c865552691},
intrahash = {2d8d00d9f4f0c5e6937976e063837b89},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
keywords = {Bacteriological Colony Count, Factors Feces, Haemophilus Handling, Humans, Microbial Microbial, Neisseria Rectum, Sensitivity Specificity, Specimen Streptococcus Techniques, Temperature, Time Viability, and gonorrhoeae, influenzae, meningitidis, pneumoniae, swab,},
month = apr,
note = {{PMID:} 17267627},
number = 4,
pages = {1278--1283},
timestamp = {2011-03-11T10:06:35.000+0100},
title = {Survival of fastidious and nonfastidious aerobic bacteria in three bacterial transport swab systems},
url = {http://www.ncbi.nlm.nih.gov/pubmed/17267627},
volume = 45,
year = 2007
}