A multiplex PCR/DNA probe assay was used to monitor Babesia bovis, B. bigemina and Anaplasma marginale infection in cattle introduced to a Boophilus microplus-infested area in Veracruz, Mexico. Eight intact, 18-month-old, cross-bred beef cattle (four naive, Group A; four Babesia species--premunized, Group B) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (PE) to ticks. Blood sample analysis for DNA detection by the MPCR/DNA probe assay showed that Group A animals were infected with B. bovis from day 11 up to day 22 PE, requiring treatment on days 17-20. Group B animals were detected positive to B. bovis on days 17-20, did not require treatment and remained persistently infected from days 70 to 84 PE. Treatment of Group A animals delayed the infection with B. bigemina. These animals became positive to the parasite on days 63-77 PE. In contrast, Group B animals (untreated) showed B. bigemina infection on days 21-26 and 63-84 PE. One animal was positive for A. marginale infection on days 63-66 PE, the rest of the animals became so on days 80-98 PE. All infected animals required treatment with oxytetracycline. Monitoring the triple hemoparasite infection with the MPCR/DNA probe assay provided important epidemiological information. Thus, precautionary measures can be established when cattle are moved to a babesiosis/anaplasmosis risk area.
%0 Journal Article
%1 Figueroa1998
%A Figueroa, J. V.
%A Alvarez, J. A.
%A Ramos, J. A.
%A Rojas, E. E.
%A Santiago, C.
%A Mosqueda, J. J.
%A Vega, C. A.
%A Buening, G. M.
%D 1998
%J Annals of The New York Academy Of Sciences
%K Follow-UpStudies Animals parasitology Babesiosis therapeuticuse epidemiology isolation/&/purification Oxytetracycline Anaplasma Babesiabovis CattleDiseases Cattle Transportation Anaplasmosis Mexico drugtherapy/epidemiology/prevention/&/control PolymeraseChainReaction Babesia Ticks
%P 1--10
%T Bovine babesiosis and anaplasmosis follow-up on cattle relocated in an endemic area for hemoparasitic diseases.
%V 849
%X A multiplex PCR/DNA probe assay was used to monitor Babesia bovis, B. bigemina and Anaplasma marginale infection in cattle introduced to a Boophilus microplus-infested area in Veracruz, Mexico. Eight intact, 18-month-old, cross-bred beef cattle (four naive, Group A; four Babesia species--premunized, Group B) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (PE) to ticks. Blood sample analysis for DNA detection by the MPCR/DNA probe assay showed that Group A animals were infected with B. bovis from day 11 up to day 22 PE, requiring treatment on days 17-20. Group B animals were detected positive to B. bovis on days 17-20, did not require treatment and remained persistently infected from days 70 to 84 PE. Treatment of Group A animals delayed the infection with B. bigemina. These animals became positive to the parasite on days 63-77 PE. In contrast, Group B animals (untreated) showed B. bigemina infection on days 21-26 and 63-84 PE. One animal was positive for A. marginale infection on days 63-66 PE, the rest of the animals became so on days 80-98 PE. All infected animals required treatment with oxytetracycline. Monitoring the triple hemoparasite infection with the MPCR/DNA probe assay provided important epidemiological information. Thus, precautionary measures can be established when cattle are moved to a babesiosis/anaplasmosis risk area.
@article{Figueroa1998,
abstract = {A multiplex PCR/DNA probe assay was used to monitor Babesia bovis, B. bigemina and Anaplasma marginale infection in cattle introduced to a Boophilus microplus-infested area in Veracruz, Mexico. Eight intact, 18-month-old, cross-bred beef cattle (four naive, Group A; four Babesia species--premunized, Group B) were immediately exposed to ticks after arrival and were clinically monitored from day 6 to day 98 post-exposure (PE) to ticks. Blood sample analysis for DNA detection by the MPCR/DNA probe assay showed that Group A animals were infected with B. bovis from day 11 up to day 22 PE, requiring treatment on days 17-20. Group B animals were detected positive to B. bovis on days 17-20, did not require treatment and remained persistently infected from days 70 to 84 PE. Treatment of Group A animals delayed the infection with B. bigemina. These animals became positive to the parasite on days 63-77 PE. In contrast, Group B animals (untreated) showed B. bigemina infection on days 21-26 and 63-84 PE. One animal was positive for A. marginale infection on days 63-66 PE, the rest of the animals became so on days 80-98 PE. All infected animals required treatment with oxytetracycline. Monitoring the triple hemoparasite infection with the MPCR/DNA probe assay provided important epidemiological information. Thus, precautionary measures can be established when cattle are moved to a babesiosis/anaplasmosis risk area.},
added-at = {2010-01-14T17:44:17.000+0100},
author = {Figueroa, J. V. and Alvarez, J. A. and Ramos, J. A. and Rojas, E. E. and Santiago, C. and Mosqueda, J. J. and Vega, C. A. and Buening, G. M.},
biburl = {https://www.bibsonomy.org/bibtex/21601c3daf13890533fab9f10351f135f/uvesco},
file = {:Figueroa1998.pdf:PDF},
institution = {CENID-PAVET, INIFAP-SAGAR Jiutepec, Morelos, Mexico.},
interhash = {22a7a00aba01c05c93150b83b8a6e6bd},
intrahash = {1601c3daf13890533fab9f10351f135f},
journal = {Annals of The New York Academy Of Sciences},
keywords = {Follow-UpStudies Animals parasitology Babesiosis therapeuticuse epidemiology isolation/&/purification Oxytetracycline Anaplasma Babesiabovis CattleDiseases Cattle Transportation Anaplasmosis Mexico drugtherapy/epidemiology/prevention/&/control PolymeraseChainReaction Babesia Ticks},
language = {eng},
medline-pst = {ppublish},
month = Jun,
pages = {1--10},
pmid = {9668444},
timestamp = {2010-01-14T17:44:18.000+0100},
title = {Bovine babesiosis and anaplasmosis follow-up on cattle relocated in an endemic area for hemoparasitic diseases.},
volume = 849,
year = 1998
}