Exhaustion of the chloroplast protein synthesis capacity by massive
expression of a highly stable protein antibiotic
M. Oey, M. Lohse, B. Kreikemeyer, и R. Bock. Plant J, 57 (3):
436-45(февраля 2009)Oey, Melanie Lohse, Marc Kreikemeyer, Bernd Bock, Ralph Research
Support, Non-U.S. Gov't England The Plant journal : for cell and
molecular biology Plant J. 2009 Feb;57(3):436-45. Epub 2008 Oct 30..
Аннотация
Plastids (chloroplasts) possess an enormous capacity to synthesize
and accumulate foreign proteins. Here we have maximized chloroplast
protein production by over-expressing a proteinaceous antibiotic
against pathogenic group A and group B streptococci from the plastid
genome. The antibiotic, a phage lytic protein, accumulated to enormously
high levels (>70% of the plant's total soluble protein), and proved
to be extremely stable in chloroplasts. This massive over-expression
exhausted the protein synthesis capacity of the chloroplast such
that the production of endogenous plastid-encoded proteins was severely
compromised. Our data suggest that this is due to translational rather
than transcriptional limitation of gene expression. We also show
that the chloroplast-produced protein antibiotic efficiently kills
the target bacteria. These unrivaled expression levels, together
with the chloroplast's insensitivity to enzymes that degrade bacterial
cell walls and the elimination of the need to remove bacterial endotoxins
by costly purification procedures, indicate that this is an effective
plant-based production platform for next-generation antibiotics,
which are urgently required to keep pace with rapidly emerging bacterial
resistance.
Oey, Melanie Lohse, Marc Kreikemeyer, Bernd Bock, Ralph Research
Support, Non-U.S. Gov't England The Plant journal : for cell and
molecular biology Plant J. 2009 Feb;57(3):436-45. Epub 2008 Oct 30.
%0 Journal Article
%1 Oey2009
%A Oey, M.
%A Lohse, M.
%A Kreikemeyer, B.
%A Bock, R.
%D 2009
%J Plant J
%K *Protein Agents/*biosynthesis/pharmacology Anti-Bacterial Biosynthesis Chloroplast Chloroplasts/genetics/*metabolism Enzymes/genetics/*metabolism Expression Gene Genetic Genetically Genome, Modified/genetics/*metabolism Plant Plants, Regulation, Streptococcus Tobacco/genetics/metabolism Transformation, effects pyogenes/drug
%N 3
%P 436-45
%T Exhaustion of the chloroplast protein synthesis capacity by massive
expression of a highly stable protein antibiotic
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18939966
%V 57
%X Plastids (chloroplasts) possess an enormous capacity to synthesize
and accumulate foreign proteins. Here we have maximized chloroplast
protein production by over-expressing a proteinaceous antibiotic
against pathogenic group A and group B streptococci from the plastid
genome. The antibiotic, a phage lytic protein, accumulated to enormously
high levels (>70% of the plant's total soluble protein), and proved
to be extremely stable in chloroplasts. This massive over-expression
exhausted the protein synthesis capacity of the chloroplast such
that the production of endogenous plastid-encoded proteins was severely
compromised. Our data suggest that this is due to translational rather
than transcriptional limitation of gene expression. We also show
that the chloroplast-produced protein antibiotic efficiently kills
the target bacteria. These unrivaled expression levels, together
with the chloroplast's insensitivity to enzymes that degrade bacterial
cell walls and the elimination of the need to remove bacterial endotoxins
by costly purification procedures, indicate that this is an effective
plant-based production platform for next-generation antibiotics,
which are urgently required to keep pace with rapidly emerging bacterial
resistance.
@article{Oey2009,
abstract = {Plastids (chloroplasts) possess an enormous capacity to synthesize
and accumulate foreign proteins. Here we have maximized chloroplast
protein production by over-expressing a proteinaceous antibiotic
against pathogenic group A and group B streptococci from the plastid
genome. The antibiotic, a phage lytic protein, accumulated to enormously
high levels (>70% of the plant's total soluble protein), and proved
to be extremely stable in chloroplasts. This massive over-expression
exhausted the protein synthesis capacity of the chloroplast such
that the production of endogenous plastid-encoded proteins was severely
compromised. Our data suggest that this is due to translational rather
than transcriptional limitation of gene expression. We also show
that the chloroplast-produced protein antibiotic efficiently kills
the target bacteria. These unrivaled expression levels, together
with the chloroplast's insensitivity to enzymes that degrade bacterial
cell walls and the elimination of the need to remove bacterial endotoxins
by costly purification procedures, indicate that this is an effective
plant-based production platform for next-generation antibiotics,
which are urgently required to keep pace with rapidly emerging bacterial
resistance.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Oey, M. and Lohse, M. and Kreikemeyer, B. and Bock, R.},
biburl = {https://www.bibsonomy.org/bibtex/216e5495c61c869f094a4613564efca8e/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {e781255ccfbb1198ec0e0ec1bd99f0cb},
intrahash = {16e5495c61c869f094a4613564efca8e},
issn = {1365-313X (Electronic) 1365-313X (Linking)},
journal = {Plant J},
keywords = {*Protein Agents/*biosynthesis/pharmacology Anti-Bacterial Biosynthesis Chloroplast Chloroplasts/genetics/*metabolism Enzymes/genetics/*metabolism Expression Gene Genetic Genetically Genome, Modified/genetics/*metabolism Plant Plants, Regulation, Streptococcus Tobacco/genetics/metabolism Transformation, effects pyogenes/drug},
month = Feb,
note = {Oey, Melanie Lohse, Marc Kreikemeyer, Bernd Bock, Ralph Research
Support, Non-U.S. Gov't England The Plant journal : for cell and
molecular biology Plant J. 2009 Feb;57(3):436-45. Epub 2008 Oct 30.},
number = 3,
pages = {436-45},
shorttitle = {Exhaustion of the chloroplast protein synthesis capacity by massive
expression of a highly stable protein antibiotic},
timestamp = {2010-12-14T18:12:27.000+0100},
title = {Exhaustion of the chloroplast protein synthesis capacity by massive
expression of a highly stable protein antibiotic},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=18939966},
volume = 57,
year = 2009
}