Several studies have been directed to development of reactors for
chemical-biological reactions have been carried out successfully. ``Dead
zones'' are areas where the substrate has non-contact with catalyst,
and this situation in reactors has been seen as inconvenience in
relation to productivity. Moreover, a catalyst immobilized in porous
support also must be difficult. We have been obtained preliminary
results in batch system of invertase immobilized on glass-ceramic
support (GCS) (1000 degrees C/3 h), were the optimum pH and temperature
of free and immobilized enzyme was similar, 5.0 and 45 degrees C. The
apparent K-m for free invertase was 13.53 times higher than the free
invertase. Aiming the work using packed bed reactors to study the
activity of invertase in different flow systems, was obtained sigmoid
curves using reactors 1 and 2, with the best result 4.75 U/g GCS and
3.09 U/g GCS, respectively. The lab-scale setups from flow system
continuous, down-flow, up-flow and alternate-flow were studied.
Alternate-flow is the alternation of down-flow and up-flow in previous
time mentioned. This innovative flow system has facilitated contact
among sucrose solution and immobilized enzyme to obtain the invert sugar
production 24 h faster, due elimination of ``dead zones'' in the
reactor in comparison to others flow systems. (C) 2012 Elsevier B.V. All
rights reserved.
%0 Journal Article
%1 WOS:000304631700001
%A Albertini, A V P
%A Reis, A L S
%A Teles, F R R
%A Souza, J C
%A Filho, J L Rolim
%A Freire, V N
%A Santos, R P
%A Martins, J L
%A Cavada, B S
%A Martins, D B G
%A Martinez, C R
%A Filho, J L Lima
%C PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS
%D 2012
%I ELSEVIER SCIENCE BV
%J JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC
%K Alternate-flow} Ceramic; Immobilized Reactor; Sintering; ashes; enzyme; fly {Coal
%P 1-7
%R 10.1016/j.molcatb.2012.04.001
%T The new flow system approach in packed bed reactor applicable for
immobilized enzyme
%V 79
%X Several studies have been directed to development of reactors for
chemical-biological reactions have been carried out successfully. ``Dead
zones'' are areas where the substrate has non-contact with catalyst,
and this situation in reactors has been seen as inconvenience in
relation to productivity. Moreover, a catalyst immobilized in porous
support also must be difficult. We have been obtained preliminary
results in batch system of invertase immobilized on glass-ceramic
support (GCS) (1000 degrees C/3 h), were the optimum pH and temperature
of free and immobilized enzyme was similar, 5.0 and 45 degrees C. The
apparent K-m for free invertase was 13.53 times higher than the free
invertase. Aiming the work using packed bed reactors to study the
activity of invertase in different flow systems, was obtained sigmoid
curves using reactors 1 and 2, with the best result 4.75 U/g GCS and
3.09 U/g GCS, respectively. The lab-scale setups from flow system
continuous, down-flow, up-flow and alternate-flow were studied.
Alternate-flow is the alternation of down-flow and up-flow in previous
time mentioned. This innovative flow system has facilitated contact
among sucrose solution and immobilized enzyme to obtain the invert sugar
production 24 h faster, due elimination of ``dead zones'' in the
reactor in comparison to others flow systems. (C) 2012 Elsevier B.V. All
rights reserved.
@article{WOS:000304631700001,
abstract = {Several studies have been directed to development of reactors for
chemical-biological reactions have been carried out successfully. ``Dead
zones'' are areas where the substrate has non-contact with catalyst,
and this situation in reactors has been seen as inconvenience in
relation to productivity. Moreover, a catalyst immobilized in porous
support also must be difficult. We have been obtained preliminary
results in batch system of invertase immobilized on glass-ceramic
support (GCS) (1000 degrees C/3 h), were the optimum pH and temperature
of free and immobilized enzyme was similar, 5.0 and 45 degrees C. The
apparent K-m for free invertase was 13.53 times higher than the free
invertase. Aiming the work using packed bed reactors to study the
activity of invertase in different flow systems, was obtained sigmoid
curves using reactors 1 and 2, with the best result 4.75 U/g GCS and
3.09 U/g GCS, respectively. The lab-scale setups from flow system
continuous, down-flow, up-flow and alternate-flow were studied.
Alternate-flow is the alternation of down-flow and up-flow in previous
time mentioned. This innovative flow system has facilitated contact
among sucrose solution and immobilized enzyme to obtain the invert sugar
production 24 h faster, due elimination of ``dead zones'' in the
reactor in comparison to others flow systems. (C) 2012 Elsevier B.V. All
rights reserved.},
added-at = {2022-05-23T20:00:14.000+0200},
address = {PO BOX 211, 1000 AE AMSTERDAM, NETHERLANDS},
author = {Albertini, A V P and Reis, A L S and Teles, F R R and Souza, J C and Filho, J L Rolim and Freire, V N and Santos, R P and Martins, J L and Cavada, B S and Martins, D B G and Martinez, C R and Filho, J L Lima},
biburl = {https://www.bibsonomy.org/bibtex/21b7ac663df5769a7eeed43bc37b8028e/ppgfis_ufc_br},
doi = {10.1016/j.molcatb.2012.04.001},
interhash = {a7c9a914c7fd15aae9eaf3e1eef18fc4},
intrahash = {1b7ac663df5769a7eeed43bc37b8028e},
issn = {1381-1177},
journal = {JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC},
keywords = {Alternate-flow} Ceramic; Immobilized Reactor; Sintering; ashes; enzyme; fly {Coal},
pages = {1-7},
publisher = {ELSEVIER SCIENCE BV},
pubstate = {published},
timestamp = {2022-05-23T20:00:14.000+0200},
title = {The new flow system approach in packed bed reactor applicable for
immobilized enzyme},
tppubtype = {article},
volume = 79,
year = 2012
}