For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.
%0 Journal Article
%1 Berthoud2009
%A Berthoud, Tamara K.
%A Dunachie, Susanna J.
%A Todryk, Stephen
%A Hill, Adrian V S.
%A Fletcher, Helen A.
%D 2009
%J J Immunol Methods
%K vaccine malaria assay
%N 1
%P 33--41
%R 10.1016/j.jim.2008.09.021
%T MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines.
%U http://dx.doi.org/10.1016/j.jim.2008.09.021
%V 340
%X For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.
@article{Berthoud2009,
abstract = {For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.},
added-at = {2012-12-19T02:26:46.000+0100},
author = {Berthoud, Tamara K. and Dunachie, Susanna J. and Todryk, Stephen and Hill, Adrian V S. and Fletcher, Helen A.},
biburl = {https://www.bibsonomy.org/bibtex/21d83336c3cc43d1de3b30dd1a75653b0/aorchid},
doi = {10.1016/j.jim.2008.09.021},
file = {:Vaccine/JImmunolMethods.340.33.pdf:PDF},
groups = {public},
institution = {University of Oxford, Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Oxford, OX3 7LJ, UK.},
interhash = {3fa2b7d9bd136b393d12c474710cbfd7},
intrahash = {1d83336c3cc43d1de3b30dd1a75653b0},
journal = {J Immunol Methods},
keywords = {vaccine malaria assay},
language = {eng},
medline-pst = {ppublish},
month = Jan,
number = 1,
pages = {33--41},
pii = {S0022-1759(08)00304-9},
pmid = {18952093},
timestamp = {2012-12-19T02:26:46.000+0100},
title = {MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines.},
url = {http://dx.doi.org/10.1016/j.jim.2008.09.021},
username = {aorchid},
volume = 340,
year = 2009
}