%0 Journal Article %1 Wang_2001_592 %A Wang, S. Q. %A Song, L. S. %A Lakatta, E. G. %A Cheng, H. %D 2001 %J Nature %K 80 Acids, Action Adrenergic, Adult, Advanced, Age Aged, Aging, Angiography, Animal, Animals, Anoxia, Aorta, Apoptosis, Arteries, Artery, Artificial, Barium, Biological Biological, Blood Brachial Bufanolides, Calcium Calcium, Cardiac Cardiomegaly, Cardiovascular Carotid Caspases, Cations, Cell Channels, Clocks, Common, Compliance, Conductivity, Congestive, Coronary DNA Dietary, Digoxin, Disease Disease, Diseases, Divalent, Doppler, Dose-Response Double-Blind Drinking, Drosophila Drug Drug, Dyes, Eating, Echocardiography, Elasticity, Electric End Estrogen Estrogens, Exercise Expression, Factors, Failure, Fatty Female, Femoral Flow Fluorescent Forecasting, Fragmentation, Gene Genome, Glycosylation Graft Heart Heart, Method, Models, Occlusion, Output, Pacing, Potentials, Pressure, Products, Rate, Relationship, Replacement Separation, Signaling, Stimulation, Test, Therapy, Tolerance, Velocity, Vessels, and melanogaster, over, %N 6828 %P 592--596 %R 10.1038/35069083 %T Ca$^2+$ signalling between single L-type Ca$^2+$ channels and ryanodine receptors in heart cells. %U http://dx.doi.org/10.1038/35069083 %V 410 %X Ca$^2+$-induced Ca$^2+$ release is a general mechanism that most cells use to amplify Ca$^2+$ signals. In heart cells, this mechanism is operated between voltage-gated L-type Ca$^2+$ channels (LCCs) in the plasma membrane and Ca$^2+$ release channels, commonly known as ryanodine receptors, in the sarcoplasmic reticulum. The Ca$^2+$ influx through LCCs traverses a cleft of roughly 12 nm formed by the cell surface and the sarcoplasmic reticulum membrane, and activates adjacent ryanodine receptors to release Ca$^2+$ in the form of Ca$^2+$ sparks. Here we determine the kinetics, fidelity and stoichiometry of coupling between LCCs and ryanodine receptors. We show that the local Ca$^2+$ signal produced by a single opening of an LCC, named a 'Ca$^2+$ sparklet', can trigger about 4-6 ryanodine receptors to generate a Ca$^2+$ spark. The coupling between LCCs and ryanodine receptors is stochastic, as judged by the exponential distribution of the coupling latency. The fraction of sparklets that successfully triggers a spark is less than unity and declines in a use-dependent manner. This optical analysis of single-channel communication affords a powerful means for elucidating Ca$^2+$-signalling mechanisms at the molecular level.

@article{Wang_2001_592, abstract = {{C}a$^{2+}$-induced {C}a$^{2+}$ release is a general mechanism that most cells use to amplify {C}a$^{2+}$ signals. In heart cells, this mechanism is operated between voltage-gated L-type {C}a$^{2+}$ channels (LCCs) in the plasma membrane and {C}a$^{2+}$ release channels, commonly known as ryanodine receptors, in the sarcoplasmic reticulum. The {C}a$^{2+}$ influx through LCCs traverses a cleft of roughly 12 nm formed by the cell surface and the sarcoplasmic reticulum membrane, and activates adjacent ryanodine receptors to release {C}a$^{2+}$ in the form of {C}a$^{2+}$ sparks. Here we determine the kinetics, fidelity and stoichiometry of coupling between LCCs and ryanodine receptors. We show that the local {C}a$^{2+}$ signal produced by a single opening of an LCC, named a '{C}a$^{2+}$ sparklet', can trigger about 4-6 ryanodine receptors to generate a {C}a$^{2+}$ spark. The coupling between LCCs and ryanodine receptors is stochastic, as judged by the exponential distribution of the coupling latency. The fraction of sparklets that successfully triggers a spark is less than unity and declines in a use-dependent manner. This optical analysis of single-channel communication affords a powerful means for elucidating {C}a$^{2+}$-signalling mechanisms at the molecular level.}, added-at = {2009-06-03T11:20:58.000+0200}, author = {Wang, S. Q. and Song, L. S. and Lakatta, E. G. and Cheng, H.}, biburl = {https://www.bibsonomy.org/bibtex/2255dadd7282e842e72e9ee82d332a67f/hake}, description = {The whole bibliography file I use.}, doi = {10.1038/35069083}, file = {Wang_2001_592.pdf:Wang_2001_592.pdf:PDF}, interhash = {5513f4e73bc7ab712c158edd99898602}, intrahash = {255dadd7282e842e72e9ee82d332a67f}, journal = {Nature}, key = 141, keywords = {80 Acids, Action Adrenergic, Adult, Advanced, Age Aged, Aging, Angiography, Animal, Animals, Anoxia, Aorta, Apoptosis, Arteries, Artery, Artificial, Barium, Biological Biological, Blood Brachial Bufanolides, Calcium Calcium, Cardiac Cardiomegaly, Cardiovascular Carotid Caspases, Cations, Cell Channels, Clocks, Common, Compliance, Conductivity, Congestive, Coronary DNA Dietary, Digoxin, Disease Disease, Diseases, Divalent, Doppler, Dose-Response Double-Blind Drinking, Drosophila Drug Drug, Dyes, Eating, Echocardiography, Elasticity, Electric End Estrogen Estrogens, Exercise Expression, Factors, Failure, Fatty Female, Femoral Flow Fluorescent Forecasting, Fragmentation, Gene Genome, Glycosylation Graft Heart Heart, Method, Models, Occlusion, Output, Pacing, Potentials, Pressure, Products, Rate, Relationship, Replacement Separation, Signaling, Stimulation, Test, Therapy, Tolerance, Velocity, Vessels, and melanogaster, over,}, month = Mar, number = 6828, pages = {592--596}, pii = {35069083}, pmid = {11279498}, timestamp = {2009-06-03T11:21:36.000+0200}, title = {{C}a$^{2+}$ signalling between single {L}-type {C}a$^{2+}$ channels and ryanodine receptors in heart cells.}, url = {http://dx.doi.org/10.1038/35069083}, volume = 410, year = 2001 }