%0 Journal Article %1 C8LC00403J %A Hassanzadeh-Barforoushi, Amin %A Law, Andrew M. K. %A Hejri, Abbas %A Asadnia, Mohsen %A Ormandy, Christopher J. %A Gallego-Ortega, David %A Ebrahimi Warkiani, Majid %D 2018 %I The Royal Society of Chemistry %J Lab Chip %K 76b45-capillarity 76t10-liquid-gas-two-phase-flows-bubbly-flows microhydraulics %N 15 %P 2156-2166 %R 10.1039/C8LC00403J %T Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment %U http://dx.doi.org/10.1039/C8LC00403J %V 18 %X We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques, the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes, is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture, image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays.

@article{C8LC00403J, abstract = {We present here a new method to easily and reliably generate an array of hundreds of dispersed nanoliter-volume semi-droplets for single-cells culture and analysis. The liquid segmentation step occurs directly in indexed traps by a tweezer-like mechanism and is stabilized by spatial confinement. Unlike common droplet-based techniques{,} the semi-droplet wets its surrounding trap walls thus supporting the culturing of both adherent and non-adherent cells. To eliminate cross-droplet cell migration and chemical cross-talk each semi-droplet is separated from a nearby trap by an ∼80 pL air plug. The overall setup and injection procedure takes less than 10 minutes{,} is insensitive to fabrication defects and supports cell recovery for downstream analysis. The method offers a new approach to easily capture{,} image and culture single cells in a chemically isolated microenvironment as a preliminary step towards high-throughput single-cell assays.}, added-at = {2019-07-11T07:28:09.000+0200}, author = {Hassanzadeh-Barforoushi, Amin and Law, Andrew M. K. and Hejri, Abbas and Asadnia, Mohsen and Ormandy, Christopher J. and Gallego-Ortega, David and Ebrahimi Warkiani, Majid}, biburl = {https://www.bibsonomy.org/bibtex/24c332a6f21f330eee233e1f0725b9105/gdmcbain}, description = {Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment - Lab on a Chip (RSC Publishing)}, doi = {10.1039/C8LC00403J}, interhash = {9f76eb3cfa7b130ef05f5e74bd7622ec}, intrahash = {4c332a6f21f330eee233e1f0725b9105}, journal = {Lab Chip}, keywords = {76b45-capillarity 76t10-liquid-gas-two-phase-flows-bubbly-flows microhydraulics}, number = 15, pages = {2156-2166}, publisher = {The Royal Society of Chemistry}, timestamp = {2019-07-11T07:28:09.000+0200}, title = {Static droplet array for culturing single live adherent cells in an isolated chemical microenvironment}, url = {http://dx.doi.org/10.1039/C8LC00403J}, volume = 18, year = 2018 }