%0 Journal Article %1 Rinaudo2007Universal %A Rinaudo, Keller %A Bleris, Leonidas %A Maddamsetti, Rohan %A Subramanian, Sairam %A Weiss, Ron %A Benenson, Yaakov %D 2007 %I Nature Publishing Group %J Nature Biotechnology %K logic rnai synthetic-biology tarpol %N 7 %P 795--801 %R 10.1038/nbt1307 %T A universal RNAi-based logic evaluator that operates in mammalian cells %U http://dx.doi.org/10.1038/nbt1307 %V 25 %X Molecular automata1, 2, 3 that combine sensing4, 5, 6, computation7, 8, 9, 10, 11, 12 and actuation13, 14 enable programmable manipulation of biological systems. We use RNA interference (RNAi)15 in human kidney cells to construct a molecular computing core that implements general Boolean logic1, 3, 8, 9, 10, 11, 12, 16 to make decisions based on endogenous molecular inputs. The state of an endogenous input is encoded by the presence or absence of 'mediator' small interfering RNAs (siRNAs). The encoding rules, combined with a specific arrangement of the siRNA targets in a synthetic gene network17, allow direct evaluation of any Boolean expression in standard forms using siRNAs and indirect evaluation using endogenous inputs. We demonstrate direct evaluation of expressions with up to five logic variables. Implementation of the encoding rules through sensory up- and down-regulatory links between the inputs and siRNA mediators will allow arbitrary Boolean decision-making using these inputs.

@article{Rinaudo2007Universal, abstract = {Molecular automata1, 2, 3 that combine sensing4, 5, 6, computation7, 8, 9, 10, 11, 12 and actuation13, 14 enable programmable manipulation of biological systems. We use {RNA} interference ({RNAi})15 in human kidney cells to construct a molecular computing core that implements general Boolean logic1, 3, 8, 9, 10, 11, 12, 16 to make decisions based on endogenous molecular inputs. The state of an endogenous input is encoded by the presence or absence of 'mediator' small interfering {RNAs} ({siRNAs}). The encoding rules, combined with a specific arrangement of the {siRNA} targets in a synthetic gene network17, allow direct evaluation of any Boolean expression in standard forms using {siRNAs} and indirect evaluation using endogenous inputs. We demonstrate direct evaluation of expressions with up to five logic variables. Implementation of the encoding rules through sensory up- and down-regulatory links between the inputs and {siRNA} mediators will allow arbitrary Boolean decision-making using these inputs.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Rinaudo, Keller and Bleris, Leonidas and Maddamsetti, Rohan and Subramanian, Sairam and Weiss, Ron and Benenson, Yaakov}, biburl = {https://www.bibsonomy.org/bibtex/24e23147ab6c88fb61f87c0ccec336eb7/karthikraman}, citeulike-article-id = {1445424}, citeulike-linkout-0 = {http://dx.doi.org/10.1038/nbt1307}, citeulike-linkout-1 = {http://dx.doi.org/10.1038/nbt1307}, citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/17515909}, citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=17515909}, day = 21, doi = {10.1038/nbt1307}, interhash = {43212292dc90817df151c8c38d8587a9}, intrahash = {4e23147ab6c88fb61f87c0ccec336eb7}, issn = {1087-0156}, journal = {Nature Biotechnology}, keywords = {logic rnai synthetic-biology tarpol}, month = may, number = 7, pages = {795--801}, pmid = {17515909}, posted-at = {2010-09-29 17:00:56}, priority = {2}, publisher = {Nature Publishing Group}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {A universal {RNAi}-based logic evaluator that operates in mammalian cells}, url = {http://dx.doi.org/10.1038/nbt1307}, volume = 25, year = 2007 }