To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99\% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.
%0 Journal Article
%1 Qin2010Human
%A Qin, Junjie
%A Li, Ruiqiang
%A Raes, Jeroen
%A Arumugam, Manimozhiyan
%A Burgdorf, Kristoffer Solvsten S.
%A Manichanh, Chaysavanh
%A Nielsen, Trine
%A Pons, Nicolas
%A Levenez, Florence
%A Yamada, Takuji
%A Mende, Daniel R.
%A Li, Junhua
%A Xu, Junming
%A Li, Shaochuan
%A Li, Dongfang
%A Cao, Jianjun
%A Wang, Bo
%A Liang, Huiqing
%A Zheng, Huisong
%A Xie, Yinlong
%A Tap, Julien
%A Lepage, Patricia
%A Bertalan, Marcelo
%A Batto, Jean-Michel M.
%A Hansen, Torben
%A Le Paslier, Denis
%A Linneberg, Allan
%A Nielsen, H. Bjørn
%A Pelletier, Eric
%A Renault, Pierre
%A Sicheritz-Ponten, Thomas
%A Turner, Keith
%A Zhu, Hongmei
%A Yu, Chang
%A Li, Shengting
%A Jian, Min
%A Zhou, Yan
%A Li, Yingrui
%A Zhang, Xiuqing
%A Li, Songgang
%A Qin, Nan
%A Yang, Huanming
%A Wang, Jian
%A Brunak, Søren
%A Doré, Joel
%A Guarner, Francisco
%A Kristiansen, Karsten
%A Pedersen, Oluf
%A Parkhill, Julian
%A Weissenbach, Jean
%A MetaHIT Consortium,
%A Bork, Peer
%A Ehrlich, S. Dusko
%A Wang, Jun
%D 2010
%I Nature Publishing Group
%J Nature
%K gut-microbiome metagenomics
%N 7285
%P 59--65
%R 10.1038/nature08821
%T A human gut microbial gene catalogue established by metagenomic sequencing.
%U http://dx.doi.org/10.1038/nature08821
%V 464
%X To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99\% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.
@article{Qin2010Human,
abstract = {To understand the impact of gut microbes on human health and well-being it is crucial to assess their genetic potential. Here we describe the Illumina-based metagenomic sequencing, assembly and characterization of 3.3 million non-redundant microbial genes, derived from 576.7 gigabases of sequence, from faecal samples of 124 European individuals. The gene set, approximately 150 times larger than the human gene complement, contains an overwhelming majority of the prevalent (more frequent) microbial genes of the cohort and probably includes a large proportion of the prevalent human intestinal microbial genes. The genes are largely shared among individuals of the cohort. Over 99\% of the genes are bacterial, indicating that the entire cohort harbours between 1,000 and 1,150 prevalent bacterial species and each individual at least 160 such species, which are also largely shared. We define and describe the minimal gut metagenome and the minimal gut bacterial genome in terms of functions present in all individuals and most bacteria, respectively.},
added-at = {2018-12-02T16:09:07.000+0100},
author = {Qin, Junjie and Li, Ruiqiang and Raes, Jeroen and Arumugam, Manimozhiyan and Burgdorf, Kristoffer Solvsten S. and Manichanh, Chaysavanh and Nielsen, Trine and Pons, Nicolas and Levenez, Florence and Yamada, Takuji and Mende, Daniel R. and Li, Junhua and Xu, Junming and Li, Shaochuan and Li, Dongfang and Cao, Jianjun and Wang, Bo and Liang, Huiqing and Zheng, Huisong and Xie, Yinlong and Tap, Julien and Lepage, Patricia and Bertalan, Marcelo and Batto, Jean-Michel M. and Hansen, Torben and Le Paslier, Denis and Linneberg, Allan and Nielsen, H. Bj{\o}rn and Pelletier, Eric and Renault, Pierre and Sicheritz-Ponten, Thomas and Turner, Keith and Zhu, Hongmei and Yu, Chang and Li, Shengting and Jian, Min and Zhou, Yan and Li, Yingrui and Zhang, Xiuqing and Li, Songgang and Qin, Nan and Yang, Huanming and Wang, Jian and Brunak, S{\o}ren and Dor\'{e}, Joel and Guarner, Francisco and Kristiansen, Karsten and Pedersen, Oluf and Parkhill, Julian and Weissenbach, Jean and {MetaHIT Consortium} and Bork, Peer and Ehrlich, S. Dusko and Wang, Jun},
biburl = {https://www.bibsonomy.org/bibtex/260bd86ee8f38c779a200a80c965001e9/karthikraman},
citeulike-article-id = {6758396},
citeulike-linkout-0 = {http://dx.doi.org/10.1038/nature08821},
citeulike-linkout-1 = {http://dx.doi.org/10.1038/nature08821},
citeulike-linkout-2 = {http://view.ncbi.nlm.nih.gov/pubmed/20203603},
citeulike-linkout-3 = {http://www.hubmed.org/display.cgi?uids=20203603},
day = 04,
doi = {10.1038/nature08821},
interhash = {0f662518567adf074229080bb4321588},
intrahash = {60bd86ee8f38c779a200a80c965001e9},
issn = {1476-4687},
journal = {Nature},
keywords = {gut-microbiome metagenomics},
month = mar,
number = 7285,
pages = {59--65},
pmid = {20203603},
posted-at = {2010-03-05 10:40:19},
priority = {2},
publisher = {Nature Publishing Group},
timestamp = {2018-12-02T16:09:07.000+0100},
title = {A human gut microbial gene catalogue established by metagenomic sequencing.},
url = {http://dx.doi.org/10.1038/nature08821},
volume = 464,
year = 2010
}