%0 Journal Article %1 jellali2016analysis %A Jellali, Rachid %A Duval, Jean-Luc %A Leclerc, Eric %D 2016 %J Materials Science and Engineering: C %K fabrication methods mold nextgen pfpe %P 295--02 %R http://dx.doi.org/10.1016/j.msec.2016.04.057 %T Analysis of the biocompatibility of perfluoropolyether dimethacrylate network using an organotypic method %U http://www.sciencedirect.com/science/article/pii/S0928493116303551 %V 65 %X Abstract In this work, we have investigated the potential of perfluoropolyether (PFPE) polymers for use in biomaterial applications, especially in cell culture and tissue engineering. \PFPE\ substrates were synthesized by the photocuring of liquid \PFPE\ urethane dimethacrylate. These surfaces were then modified by \ECM\ protein coatings and microstructuration, to promote cell adhesion and migration. The surface properties of \PFPE\ and \PDMS\ (used as a reference) samples were studied by static contact angle measurements and \AFM\ imaging. Both polymer surfaces were hydrophobic, having sessile air–water contact angles superior to 100°. Collagen and fibronectin coatings were found to change the wettability of \PFPE\ and \PDMS\ samples. The biological testing of substrates was done using a liver organotypic culture to evaluate the migration and density of liver cells. The results over seven days of culture demonstrated that the migration and density of cells cultured under untreated \PFPE\ were higher than the migration and density of cells cultured under PDMS. \ECM\ protein coatings enhanced cell migration from liver explants cultured on \PFPE\ or PDMS. Furthermore, these coatings were more efficient in the case of a \PFPE\ sample. From a second series of tests, in which the \PFPE\ was microstructured, it was found that microstructures promoted the formation of a 3D cell layer. These results indicate that \PFPE\ polymers have a potential for use in the development of biomaterials for tissue engineering and cell culture.

@article{jellali2016analysis, abstract = {Abstract In this work, we have investigated the potential of perfluoropolyether (PFPE) polymers for use in biomaterial applications, especially in cell culture and tissue engineering. \{PFPE\} substrates were synthesized by the photocuring of liquid \{PFPE\} urethane dimethacrylate. These surfaces were then modified by \{ECM\} protein coatings and microstructuration, to promote cell adhesion and migration. The surface properties of \{PFPE\} and \{PDMS\} (used as a reference) samples were studied by static contact angle measurements and \{AFM\} imaging. Both polymer surfaces were hydrophobic, having sessile air–water contact angles superior to 100°. Collagen and fibronectin coatings were found to change the wettability of \{PFPE\} and \{PDMS\} samples. The biological testing of substrates was done using a liver organotypic culture to evaluate the migration and density of liver cells. The results over seven days of culture demonstrated that the migration and density of cells cultured under untreated \{PFPE\} were higher than the migration and density of cells cultured under PDMS. \{ECM\} protein coatings enhanced cell migration from liver explants cultured on \{PFPE\} or PDMS. Furthermore, these coatings were more efficient in the case of a \{PFPE\} sample. From a second series of tests, in which the \{PFPE\} was microstructured, it was found that microstructures promoted the formation of a 3D cell layer. These results indicate that \{PFPE\} polymers have a potential for use in the development of biomaterials for tissue engineering and cell culture. }, added-at = {2016-05-11T13:20:06.000+0200}, author = {Jellali, Rachid and Duval, Jean-Luc and Leclerc, Eric}, biburl = {https://www.bibsonomy.org/bibtex/26cc483e2a04ca464e1d24e461ac620a3/bkoch}, description = {Analysis of the biocompatibility of perfluoropolyether dimethacrylate network using an organotypic method}, doi = {http://dx.doi.org/10.1016/j.msec.2016.04.057}, interhash = {36a756b7e10926c1e16d90bc3d4554b7}, intrahash = {6cc483e2a04ca464e1d24e461ac620a3}, issn = {0928-4931}, journal = {Materials Science and Engineering: C }, keywords = {fabrication methods mold nextgen pfpe}, pages = {295--02}, timestamp = {2016-05-11T13:20:36.000+0200}, title = {Analysis of the biocompatibility of perfluoropolyether dimethacrylate network using an organotypic method }, url = {http://www.sciencedirect.com/science/article/pii/S0928493116303551}, volume = 65, year = 2016 }