The complete gene cluster for biosynthesis of a polyene complex, FR-008,
spans 137.2 kb of the genome of Streptomyces sp. FR-008 consisting
of six genes for a modular PKS and 15 additional genes. The extensive
similarity to the partially characterized candicidin gene cluster
in Streptomyces griseus IMRU3570, especially for genes involved
in mycosamine biosynthesis, prompted us to compare the compounds
produced by Streptomyces sp. FR-008 and Streptomyces griseus IMRU3570,
and we found that FR-008 and candicidin complex are identical. A
model for biosynthesis of a set of four structurally related FR-008/candicidin
compounds was proposed. Deletion of the putative regulatory genes
abolished antibiotic production, while disruption of putative glycosyltransferase
and GDP-ketosugar aminotransferase functionalities led to the productions
of a set of nonmycosaminated aglycones and a novel polyene complex
with attachment of altered sugar moiety, respectively.
%0 Journal Article
%1 citeulike:952947
%A Chen, S.
%A Huang, X.
%A Zhou, X.
%A Bai, L.
%A He, J.
%A Jeong, K. J.
%A Lee, S. Y.
%A Deng, Z.
%C Bio-X Life Science Research Center and School of Life Science and
Biotechnology, Shanghai Jiaotong University, 200030, Shanghai, China.
%D 2003
%J Chem Biol
%K paba-synthase
%N 11
%P 1065--1076
%T Organizational and mutational analysis of a complete FR-008/candicidin
gene cluster encoding a structurally related polyene complex.
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=14652074
%V 10
%X The complete gene cluster for biosynthesis of a polyene complex, FR-008,
spans 137.2 kb of the genome of Streptomyces sp. FR-008 consisting
of six genes for a modular PKS and 15 additional genes. The extensive
similarity to the partially characterized candicidin gene cluster
in Streptomyces griseus IMRU3570, especially for genes involved
in mycosamine biosynthesis, prompted us to compare the compounds
produced by Streptomyces sp. FR-008 and Streptomyces griseus IMRU3570,
and we found that FR-008 and candicidin complex are identical. A
model for biosynthesis of a set of four structurally related FR-008/candicidin
compounds was proposed. Deletion of the putative regulatory genes
abolished antibiotic production, while disruption of putative glycosyltransferase
and GDP-ketosugar aminotransferase functionalities led to the productions
of a set of nonmycosaminated aglycones and a novel polyene complex
with attachment of altered sugar moiety, respectively.
@article{citeulike:952947,
abstract = {The complete gene cluster for biosynthesis of a polyene complex, FR-008,
spans 137.2 kb of the genome of Streptomyces sp. FR-008 consisting
of six genes for a modular PKS and 15 additional genes. The extensive
similarity to the partially characterized candicidin gene cluster
in Streptomyces griseus IMRU3570, especially for genes involved
in mycosamine biosynthesis, prompted us to compare the compounds
produced by Streptomyces sp. FR-008 and Streptomyces griseus IMRU3570,
and we found that FR-008 and candicidin complex are identical. A
model for biosynthesis of a set of four structurally related FR-008/candicidin
compounds was proposed. Deletion of the putative regulatory genes
abolished antibiotic production, while disruption of putative glycosyltransferase
and GDP-ketosugar aminotransferase functionalities led to the productions
of a set of nonmycosaminated aglycones and a novel polyene complex
with attachment of altered sugar moiety, respectively.},
added-at = {2007-02-02T11:54:15.000+0100},
address = {Bio-X Life Science Research Center and School of Life Science and
Biotechnology, Shanghai Jiaotong University, 200030, Shanghai, China.},
author = {Chen, S. and Huang, X. and Zhou, X. and Bai, L. and He, J. and Jeong, K. J. and Lee, S. Y. and Deng, Z.},
biburl = {https://www.bibsonomy.org/bibtex/26e45ebb43af4c1deca4a912a302028a5/robert},
citeulike-article-id = {952947},
interhash = {fb658a887acb0c18933d763a853ebbcb},
intrahash = {6e45ebb43af4c1deca4a912a302028a5},
issn = {1074-5521},
journal = {Chem Biol},
keywords = {paba-synthase},
month = {November},
number = 11,
pages = {1065--1076},
priority = {2},
timestamp = {2007-02-02T11:54:15.000+0100},
title = {Organizational and mutational analysis of a complete FR-008/candicidin
gene cluster encoding a structurally related polyene complex.},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=14652074},
volume = 10,
year = 2003
}