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Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform

, , and . Cold Spring Harb Protoc 2015 (7): 626-633 (March 2015)

Abstract

There are multiple platforms available for whole-exome enrichment and sequencing (WES). This protocol is based on the Agilent SureSelect Human All Exon platform, which targets ∼50 Mb of the human exonic regions. The SureSelect system uses ∼120-base RNA probes to capture known coding DNA sequences (CDS) from the NCBI Consensus CDS Database as well as other major RNA coding sequence databases, such as Sanger miRBase. The protocol can be performed at the benchside without the need for automation, and the resulting library can be used for targeted next-generation sequencing on an Illumina HiSeq 2000 sequencer.

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Whole-Exome Enrichment with the Agilent SureSelect Human All Exon Platform

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Chen:2015:Cold-Spring-Harb-Protoc:25762417
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