%0 Journal Article %1 das_dipolar_2014 %A Das, Bibhuti B %A Zhang, Hua %A Opella, Stanley J %D 2014 %J J. Magn. Reson. %K Algorithms,Lipid NMR,Vpu Proteins,Membrane Resonance Results,Sensitivity Specificity,Solid Spectroscopy,Membrane and bilayers,MAS,Magnetic of protein,Phospholipids,Reproducibility state %P 224--232 %R 10.1016/j.jmr.2014.02.018 %T Dipolar \Assisted\ \Assignment\ \Protocol\ (\DAAP\) for \MAS\ solid-state \NMR\ of rotationally aligned membrane proteins in phospholipid bilayers %V 242 %X A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of heteronuclear dipolar coupling frequencies in combination with conventional chemical shift based assignment methods is demonstrated. The Dipolar Assisted Assignment Protocol (DAAP) takes advantage of the rotational alignment of the membrane proteins in liquid crystalline phospholipid bilayers. Improved resolution is obtained by combining the magnetically inequivalent heteronuclear dipolar frequencies with isotropic chemical shift frequencies. Spectra with both dipolar and chemical shift frequency axes assist with resonance assignments. DAAP can be readily extended to three- and four-dimensional experiments and to include both backbone and side chain sites in proteins.

@article{das_dipolar_2014, abstract = {A method for making resonance assignments in magic angle spinning solid-state NMR spectra of membrane proteins that utilizes the range of heteronuclear dipolar coupling frequencies in combination with conventional chemical shift based assignment methods is demonstrated. The Dipolar Assisted Assignment Protocol (DAAP) takes advantage of the rotational alignment of the membrane proteins in liquid crystalline phospholipid bilayers. Improved resolution is obtained by combining the magnetically inequivalent heteronuclear dipolar frequencies with isotropic chemical shift frequencies. Spectra with both dipolar and chemical shift frequency axes assist with resonance assignments. DAAP can be readily extended to three- and four-dimensional experiments and to include both backbone and side chain sites in proteins.}, added-at = {2017-03-14T02:48:56.000+0100}, author = {Das, Bibhuti B and Zhang, Hua and Opella, Stanley J}, biburl = {https://www.bibsonomy.org/bibtex/27b41f70ee5b8846ec9b5649a05a194ae/nmrresource}, doi = {10.1016/j.jmr.2014.02.018}, interhash = {1ce631493066452d24f8cec330189582}, intrahash = {7b41f70ee5b8846ec9b5649a05a194ae}, issn = {1096-0856}, journal = {J. Magn. Reson.}, keywords = {Algorithms,Lipid NMR,Vpu Proteins,Membrane Resonance Results,Sensitivity Specificity,Solid Spectroscopy,Membrane and bilayers,MAS,Magnetic of protein,Phospholipids,Reproducibility state}, month = may, pages = {224--232}, pmid = {24698983}, timestamp = {2017-03-14T02:49:21.000+0100}, title = {{Dipolar {\{}Assisted{\}} {\{}Assignment{\}} {\{}Protocol{\}} ({\{}DAAP{\}}) for {\{}MAS{\}} solid-state {\{}NMR{\}} of rotationally aligned membrane proteins in phospholipid bilayers}}, volume = 242, year = 2014 }