%0 Journal Article %1 Xu2012 %A Xu, Ke %A Babcock, Hazen P. %A Zhuang, Xiaowei %D 2012 %I Nature Publishing Group %J Nature Methods %K 3D actin biology cytoskeleton microscopy neuro storm superresolution %N 2 %P 185--188 %R 10.1038/nmeth.1841 %T Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton %V 9 %X By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained \textless10-nm lateral resolution and \textless20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions. © 2012 Nature America, Inc. All rights reserved.

@article{Xu2012, abstract = {By combining astigmatism imaging with a dual-objective scheme, we improved the image resolution of stochastic optical reconstruction microscopy (STORM) and obtained {\textless}10-nm lateral resolution and {\textless}20-nm axial resolution when imaging biological specimens. Using this approach, we resolved individual actin filaments in cells and revealed three-dimensional ultrastructure of the actin cytoskeleton. We observed two vertically separated layers of actin networks with distinct structural organizations in sheet-like cell protrusions. {\textcopyright} 2012 Nature America, Inc. All rights reserved.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Xu, Ke and Babcock, Hazen P. and Zhuang, Xiaowei}, biburl = {https://www.bibsonomy.org/bibtex/27db9cbb8d00aef74481fac982678a157/kfriedl}, doi = {10.1038/nmeth.1841}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd/Mendeley Desktop/Downloaded/dualobjectivestormzhuangx.pdf:pdf}, interhash = {e140bfb8726db90657be34ef2205cd2c}, intrahash = {7db9cbb8d00aef74481fac982678a157}, issn = {15487091}, journal = {Nature Methods}, keywords = {3D actin biology cytoskeleton microscopy neuro storm superresolution}, month = feb, number = 2, pages = {185--188}, publisher = {Nature Publishing Group}, timestamp = {2020-04-07T11:00:13.000+0200}, title = {{Dual-objective STORM reveals three-dimensional filament organization in the actin cytoskeleton}}, volume = 9, year = 2012 }