Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7\%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7\%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
%0 Journal Article
%1 taha_interlaboratory_2005
%A Taha, Muhamed-Kheir
%A Alonso, Jean-Michel
%A Cafferkey, Mary
%A Caugant, Dominique A
%A Clarke, Stuart C
%A Diggle, Mathew A
%A Fox, Andrew
%A Frosch, Matthias
%A Gray, Stephen J
%A Guiver, Malcolm
%A Heuberger, Sigrid
%A Kalmusova, Jitka
%A Kesanopoulos, Konstantinos
%A Klem, Anne-Marie
%A Kriz, Paula
%A Marsh, John
%A MĂślling, Paula
%A Murphy, Karen
%A OlcĂŠn, Per
%A Sanou, Oumar
%A Tzanakaki, Georgina
%A Vogel, Ulrich
%D 2005
%J Journal of Clinical Microbiology
%K Adolescent, Adult, Bacterial, Burkina Chain Child, European Faso, Female, Genotype, Humans, Infant, Infections, Laboratories, Male, Meningitis, Meningococcal Meningococcal, Neisseria Polymerase Preschool, Reaction, Sensitivity Specificity Union, and meningitidis, {DNA},
%N 1
%P 144--149
%R 10.1128/JCM.43.1.144-149.2005
%T Interlaboratory comparison of PCR-based identification and genogrouping of Neisseria meningitidis
%U http://www.ncbi.nlm.nih.gov/pubmed/15634963
%V 43
%X Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the EU-MenNet program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes (crgA, ctrA, 16S rRNA, and porA). The PCR-based nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7\%, respectively. Most of the laboratories also performed genogrouping assays (siaD and mynB/sacC). The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7\%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this PCR. The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these PCRs.
@article{taha_interlaboratory_2005,
abstract = {Twenty clinical samples (18 cerebrospinal fluid samples and 2 articular fluid samples) were sent to 11 meningococcus reference centers located in 11 different countries. Ten of these laboratories are participating in the {EU-MenNet} program (a European Union-funded program) and are members of the European Monitoring Group on Meningococci. The remaining laboratory was located in Burkina Faso. Neisseria meningitidis was sought by detecting several meningococcus-specific genes {(crgA}, {ctrA}, {16S} {rRNA}, and {porA).} The {PCR-based} nonculture method for the detection of N. meningitidis gave similar results between participants with a mean sensitivity and specificity of 89.7 and 92.7\%, respectively. Most of the laboratories also performed genogrouping assays {(siaD} and {mynB/sacC).} The performance of genogrouping was more variable between laboratories, with a mean sensitivity of 72.7\%. Genogroup B gave the best correlation between participants, as all laboratories routinely perform this {PCR.} The results for genogroups A and W135 were less similar between the eight participating laboratories that performed these {PCRs.}},
added-at = {2011-06-24T11:21:47.000+0200},
author = {Taha, {Muhamed-Kheir} and Alonso, {Jean-Michel} and Cafferkey, Mary and Caugant, Dominique A and Clarke, Stuart C and Diggle, Mathew A and Fox, Andrew and Frosch, Matthias and Gray, Stephen J and Guiver, Malcolm and Heuberger, Sigrid and Kalmusova, Jitka and Kesanopoulos, Konstantinos and Klem, {Anne-Marie} and Kriz, Paula and Marsh, John and MĂślling, Paula and Murphy, Karen and OlcĂŠn, Per and Sanou, Oumar and Tzanakaki, Georgina and Vogel, Ulrich},
biburl = {https://www.bibsonomy.org/bibtex/294373fbcd064652a42587dcc3302cf03/ag_vogel},
doi = {10.1128/JCM.43.1.144-149.2005},
interhash = {3cf9d4ba0bd39e49f70c4558434a5402},
intrahash = {94373fbcd064652a42587dcc3302cf03},
issn = {0095-1137},
journal = {Journal of Clinical Microbiology},
keywords = {Adolescent, Adult, Bacterial, Burkina Chain Child, European Faso, Female, Genotype, Humans, Infant, Infections, Laboratories, Male, Meningitis, Meningococcal Meningococcal, Neisseria Polymerase Preschool, Reaction, Sensitivity Specificity Union, and meningitidis, {DNA},},
month = jan,
note = {{PMID:} 15634963},
number = 1,
pages = {144--149},
timestamp = {2011-06-24T11:21:48.000+0200},
title = {Interlaboratory comparison of {PCR-based} identification and genogrouping of Neisseria meningitidis},
url = {http://www.ncbi.nlm.nih.gov/pubmed/15634963},
volume = 43,
year = 2005
}