%0 Generic %1 sakurai2013strong %A SAKURAI, T %A HIROTA, N %D 2013 %K cellular differentiation experiment magnet myogenic superconducting %T Strong static magnetic fields induced orientation of myotubes differentiated from myoblasts %X PURPOSE: During the skeletal muscle repair processes, myoblasts differentiate into muscle cells, and the muscle cells form multinucleated myotubes. In in vitro studies, the myotubes have generally been randomly arranged. However, we recently reported that exposure to strong static magnetic fields (SMFs) at 10 T could induce the orientation of myotubes differentiated from myoblasts. In this study, we investigated the strong SMFs-induced myotube orientation using new SMF-exposure systems. MATERIALS AND METHODS: The myogenic differentiation of a mouse-derived myoblast cell line, C2C12, was conducted for six days under exposure to strong SMFs for either all six days, the initial four days or for the last two days. The magnetic flux density and the product of the magnetic flux density and magnetic field gradient at the exposed positions were as follows: (a) 13 T-0 T2/m, (b) 9 T-585 T2/m and (c) 1.5 T-22 T2/m. Exposure to SMFs was performed using an exposure system, which was developed by modifying a superconducting magnet (JMTD-10C13E-NC) generating up to 13T of vertical SMF within its 100-mmdiameter bore. The temperature within the bore was maintained at 3 7°C by running warm water in a silicon tube covering the internal surface of the magnet bore. After myogenic differentiation, the cells were fixed with 4% paraformaldehyde/phosphate buffer solutions and stained with an anti-myosin heavy chain antibody. RESULTS: Exposure to SMF at 13 T-0 T2/m position for six days led to significant formation of oriented myotubes. Exposure at 9 T-585 T2/m position led to less oriented myotube formation, and exposure at 1.5 T-22 T2/m position did not induce orientation. The degree of myotube orientation following exposure to SMFs at 13 T-0 T2/m position or 9 T-585 T2/m position for the initial four days was not obviously different from that after exposure for six days. Myotube orientation was not observed under exposure to SMFs at all positions for just the last two days of myogenic differentiation. The SMF-induced formation of oriented myotubes depends on the magnetic flux density and period of exposure.

@conference{sakurai2013strong, abstract = {PURPOSE: During the skeletal muscle repair processes, myoblasts differentiate into muscle cells, and the muscle cells form multinucleated myotubes. In in vitro studies, the myotubes have generally been randomly arranged. However, we recently reported that exposure to strong static magnetic fields (SMFs) at 10 T could induce the orientation of myotubes differentiated from myoblasts. In this study, we investigated the strong SMFs-induced myotube orientation using new SMF-exposure systems. MATERIALS AND METHODS: The myogenic differentiation of a mouse-derived myoblast cell line, C2C12, was conducted for six days under exposure to strong SMFs for either all six days, the initial four days or for the last two days. The magnetic flux density and the product of the magnetic flux density and magnetic field gradient at the exposed positions were as follows: (a) 13 T-0 T2/m, (b) 9 T-585 T2/m and (c) 1.5 T-22 T2/m. Exposure to SMFs was performed using an exposure system, which was developed by modifying a superconducting magnet (JMTD-10C13E-NC) generating up to 13T of vertical SMF within its 100-mmdiameter bore. The temperature within the bore was maintained at 3 7°C by running warm water in a silicon tube covering the internal surface of the magnet bore. After myogenic differentiation, the cells were fixed with 4% paraformaldehyde/phosphate buffer solutions and stained with an anti-myosin heavy chain antibody. RESULTS: Exposure to SMF at 13 T-0 T2/m position for six days led to significant formation of oriented myotubes. Exposure at 9 T-585 T2/m position led to less oriented myotube formation, and exposure at 1.5 T-22 T2/m position did not induce orientation. The degree of myotube orientation following exposure to SMFs at 13 T-0 T2/m position or 9 T-585 T2/m position for the initial four days was not obviously different from that after exposure for six days. Myotube orientation was not observed under exposure to SMFs at all positions for just the last two days of myogenic differentiation. The SMF-induced formation of oriented myotubes depends on the magnetic flux density and period of exposure.}, added-at = {2021-02-12T12:10:57.000+0100}, author = {SAKURAI, T and HIROTA, N}, biburl = {https://www.bibsonomy.org/bibtex/2950f5cbf6d9b105957a8298b2d818149/chkokalis}, interhash = {73ebd0ada452d39f2f943e2f81a4ad11}, intrahash = {950f5cbf6d9b105957a8298b2d818149}, keywords = {cellular differentiation experiment magnet myogenic superconducting}, timestamp = {2021-02-12T16:11:24.000+0100}, title = {Strong static magnetic fields induced orientation of myotubes differentiated from myoblasts}, year = 2013 }