%0 Journal Article %1 MOON2011175 %A Moon, Jai-Hee %A Kwon, Suhyun %A Jun, Eun Kyoung %A Kim, Aeree %A Whang, Kwang Youn %A Kim, Hyunggee %A Oh, Sejong %A Yoon, Byung Sun %A You, Seungkwon %D 2011 %J Biochemical and Biophysical Research Communications %K cancerstemcell %N 1 %P 175 - 181 %R https://doi.org/10.1016/j.bbrc.2011.07.070 %T Nanog-induced dedifferentiation of p53-deficient mouse astrocytes into brain cancer stem-like cells %U http://www.sciencedirect.com/science/article/pii/S0006291X11012964 %V 412 %X Self-renewal, differentiation, and tumorigenicity characterize cancer stem cells (CSCs), which are rare and maintained by specific cell fate regulators. CSCs are isolated from glioblastoma multiforme (GBM) and may be responsible for the lethality of incurable brain tumors. Brain CSCs may arise from the transformation of undifferentiated, nestin-positive neural stem or progenitor cells and GFAP-expressing astrocytes. Here, we report a role of Nanog in the genesis of cancer stem-like cells. Using primary murine p53-knockout astrocytes (p53−/− astrocytes), we provide evidence that enforced Nanog expression can increase the cellular growth rate and transform phenotypes in vitro and in vivo. In addition, Nanog drives p53−/− astrocytes toward a dedifferentiated, CSC-like phenotype with characteristic neural stem cell/progenitor marker expression, neurosphere formation, self-renewal activity, and tumor development. These findings suggest that Nanog promotes dedifferentiation of p53-deficient mouse astrocytes into cancer stem-like cells by changing the cell fate and transforming cell properties.

@article{MOON2011175, abstract = {Self-renewal, differentiation, and tumorigenicity characterize cancer stem cells (CSCs), which are rare and maintained by specific cell fate regulators. CSCs are isolated from glioblastoma multiforme (GBM) and may be responsible for the lethality of incurable brain tumors. Brain CSCs may arise from the transformation of undifferentiated, nestin-positive neural stem or progenitor cells and GFAP-expressing astrocytes. Here, we report a role of Nanog in the genesis of cancer stem-like cells. Using primary murine p53-knockout astrocytes (p53−/− astrocytes), we provide evidence that enforced Nanog expression can increase the cellular growth rate and transform phenotypes in vitro and in vivo. In addition, Nanog drives p53−/− astrocytes toward a dedifferentiated, CSC-like phenotype with characteristic neural stem cell/progenitor marker expression, neurosphere formation, self-renewal activity, and tumor development. These findings suggest that Nanog promotes dedifferentiation of p53-deficient mouse astrocytes into cancer stem-like cells by changing the cell fate and transforming cell properties.}, added-at = {2020-08-29T13:19:41.000+0200}, author = {Moon, Jai-Hee and Kwon, Suhyun and Jun, Eun Kyoung and Kim, Aeree and Whang, Kwang Youn and Kim, Hyunggee and Oh, Sejong and Yoon, Byung Sun and You, Seungkwon}, biburl = {https://www.bibsonomy.org/bibtex/298a8fb74a7b8b5e4c0a82106818a28e8/sam-kelly}, doi = {https://doi.org/10.1016/j.bbrc.2011.07.070}, interhash = {92d96d69d20c4d57e88cab2b0ed733e1}, intrahash = {98a8fb74a7b8b5e4c0a82106818a28e8}, issn = {0006-291X}, journal = {Biochemical and Biophysical Research Communications}, keywords = {cancerstemcell}, number = 1, pages = {175 - 181}, timestamp = {2020-08-29T13:19:41.000+0200}, title = {Nanog-induced dedifferentiation of p53-deficient mouse astrocytes into brain cancer stem-like cells}, url = {http://www.sciencedirect.com/science/article/pii/S0006291X11012964}, volume = 412, year = 2011 }