%0 Journal Article %1 RAMSKOLD2019517 %A Ramsköld, Daniel %A Parodis, Ioannis %A Lakshmikanth, Tadepally %A Sippl, Natalie %A Khademi, Mohsen %A Chen, Yang %A Zickert, Agneta %A Mikeš, Jaromír %A Achour, Adnane %A Amara, Khaled %A Piehl, Fredrik %A Brodin, Petter %A Gunnarsson, Iva %A Malmström, Vivianne %D 2019 %J EBioMedicine %K B cells %P 517 - 527 %R https://doi.org/10.1016/j.ebiom.2018.12.035 %T B cell alterations during BAFF inhibition with belimumab in SLE %U http://www.sciencedirect.com/science/article/pii/S2352396418306121 %V 40 %X Background Systemic lupus erythematosus (SLE) is a systemic autoimmune disease, which exhibits multiple B cell abnormalities including expanded populations of memory B cells and elevated levels of autoantibodies. Belimumab is a monoclonal antibody targeting the B cell cytokine BAFF (a.k.a. BLyS), approved for the treatment of SLE. Methods In this prospective cohort study, B cells from peripheral blood of 23 SLE patients initiating belimumab treatment and followed longitudinally for up to three years, were assessed using mass cytometry. Findings B cells decreased during the study period, with a rapid decrease of both naïve and CD11c+CD21− B cells at the first follow-up visit, followed by a continuous reduction at subsequent follow-ups. In contrast, plasma cells and switched memory B cells remained stable throughout the study. The observed immunological changes correlated with early, but not late, clinical improvements. Moreover, high baseline B cell counts were predictive of failure to attain low disease activity. In summary, our data unveiled both rapid and gradual later therapy-associated alterations of both known and unforeseen B cell phenotypes. Interpretation Our results suggest that evaluation of B cell counts might prove useful prior to initiation of belimumab treatment and that early treatment evaluation and discontinuation might underestimate delayed clinical improvements resultant of late B cell changes.

@article{RAMSKOLD2019517, abstract = {Background Systemic lupus erythematosus (SLE) is a systemic autoimmune disease, which exhibits multiple B cell abnormalities including expanded populations of memory B cells and elevated levels of autoantibodies. Belimumab is a monoclonal antibody targeting the B cell cytokine BAFF (a.k.a. BLyS), approved for the treatment of SLE. Methods In this prospective cohort study, B cells from peripheral blood of 23 SLE patients initiating belimumab treatment and followed longitudinally for up to three years, were assessed using mass cytometry. Findings B cells decreased during the study period, with a rapid decrease of both naïve and CD11c+CD21− B cells at the first follow-up visit, followed by a continuous reduction at subsequent follow-ups. In contrast, plasma cells and switched memory B cells remained stable throughout the study. The observed immunological changes correlated with early, but not late, clinical improvements. Moreover, high baseline B cell counts were predictive of failure to attain low disease activity. In summary, our data unveiled both rapid and gradual later therapy-associated alterations of both known and unforeseen B cell phenotypes. Interpretation Our results suggest that evaluation of B cell counts might prove useful prior to initiation of belimumab treatment and that early treatment evaluation and discontinuation might underestimate delayed clinical improvements resultant of late B cell changes.}, added-at = {2020-04-02T18:46:07.000+0200}, author = {Ramsköld, Daniel and Parodis, Ioannis and Lakshmikanth, Tadepally and Sippl, Natalie and Khademi, Mohsen and Chen, Yang and Zickert, Agneta and Mikeš, Jaromír and Achour, Adnane and Amara, Khaled and Piehl, Fredrik and Brodin, Petter and Gunnarsson, Iva and Malmström, Vivianne}, biburl = {https://www.bibsonomy.org/bibtex/299902f04d3720c09ba5232c3644025d3/lkanth}, description = {B cell alterations during BAFF inhibition with belimumab in SLE - ScienceDirect}, doi = {https://doi.org/10.1016/j.ebiom.2018.12.035}, interhash = {b646902dcbc026a4dce8a11ff076b460}, intrahash = {99902f04d3720c09ba5232c3644025d3}, issn = {2352-3964}, journal = {EBioMedicine}, keywords = {B cells}, pages = {517 - 527}, timestamp = {2020-04-02T18:46:07.000+0200}, title = {B cell alterations during BAFF inhibition with belimumab in SLE}, url = {http://www.sciencedirect.com/science/article/pii/S2352396418306121}, volume = 40, year = 2019 }