Piriformospora indica, an endophytic root-colonizing fungus, efficiently promotes plant growth and induces resistance to abiotic stress and biotic diseases. P. indica fungal cell wall extract induces cytoplasmic calcium elevation in host plant roots. Here, we show that cellotriose (CT) is an elicitor-active cell wall moiety released by P. indica into the medium. CT induces a mild defense-like response, including the production of reactive oxygen species, changes in membrane potential, and the expression of genes involved in growth regulation and root development. CT-based cytoplasmic calcium elevation in Arabidopsis (Arabidopsis thaliana) roots does not require the BAK1 coreceptor or the putative Ca(2+) channels TPC1, GLR3.3, GLR2.4, and GLR2.5 and operates synergistically with the elicitor chitin. We identified an ethyl methanesulfonate-induced mutant (cytoplasmiccalcium elevation mutant) impaired in the response to CT and various other cellooligomers (n = 2-7), but not to chitooligomers (n = 4-8), in roots. The mutant contains a single nucleotide exchange in the gene encoding a poly(A) ribonuclease (AtPARN; At1g55870) that degrades the poly(A) tails of specific mRNAs. The wild-type PARN cDNA, expressed under the control of a 35S promoter, complements the mutant phenotype. Our identification of cellotriose as a novel chemical mediator casts light on the complex P. indica-plant mutualistic relationship.
Johnson, Joy M
Thurich, Johannes
Petutschnig, Elena K
Altschmied, Lothar
Meichsner, Doreen
Sherameti, Irena
Dindas, Julian
Mrozinska, Anna
Paetz, Christian
Scholz, Sandra S
Furch, Alexandra C U
Lipka, Volker
Hedrich, Rainer
Schneider, Bernd
Svatos, Ales
Oelmuller, Ralf
eng
Research Support, Non-U.S. Gov't
2018/01/27
Plant Physiol. 2018 Mar;176(3):2496-2514. doi: 10.1104/pp.17.01423. Epub 2018 Jan 25.
%0 Journal Article
%1 johnson2018polya
%A Johnson, J. M.
%A Thurich, J.
%A Petutschnig, E. K.
%A Altschmied, L.
%A Meichsner, D.
%A Sherameti, I.
%A Dindas, J.
%A Mrozinska, A.
%A Paetz, C.
%A Scholz, S. S.
%A Furch, A. C. U.
%A Lipka, V.
%A Hedrich, R.
%A Schneider, B.
%A Svatos, A.
%A Oelmuller, R.
%D 2018
%J Plant Physiol
%K Arabidopsis/genetics/metabolism/*microbiology myOwn
%N 3
%P 2496-2514
%R 10.1104/pp.17.01423
%T A Poly(A) Ribonuclease Controls the Cellotriose-Based Interaction between Piriformospora indica and Its Host Arabidopsis
%U https://www.ncbi.nlm.nih.gov/pubmed/29371249
%V 176
%X Piriformospora indica, an endophytic root-colonizing fungus, efficiently promotes plant growth and induces resistance to abiotic stress and biotic diseases. P. indica fungal cell wall extract induces cytoplasmic calcium elevation in host plant roots. Here, we show that cellotriose (CT) is an elicitor-active cell wall moiety released by P. indica into the medium. CT induces a mild defense-like response, including the production of reactive oxygen species, changes in membrane potential, and the expression of genes involved in growth regulation and root development. CT-based cytoplasmic calcium elevation in Arabidopsis (Arabidopsis thaliana) roots does not require the BAK1 coreceptor or the putative Ca(2+) channels TPC1, GLR3.3, GLR2.4, and GLR2.5 and operates synergistically with the elicitor chitin. We identified an ethyl methanesulfonate-induced mutant (cytoplasmiccalcium elevation mutant) impaired in the response to CT and various other cellooligomers (n = 2-7), but not to chitooligomers (n = 4-8), in roots. The mutant contains a single nucleotide exchange in the gene encoding a poly(A) ribonuclease (AtPARN; At1g55870) that degrades the poly(A) tails of specific mRNAs. The wild-type PARN cDNA, expressed under the control of a 35S promoter, complements the mutant phenotype. Our identification of cellotriose as a novel chemical mediator casts light on the complex P. indica-plant mutualistic relationship.
@article{johnson2018polya,
abstract = {Piriformospora indica, an endophytic root-colonizing fungus, efficiently promotes plant growth and induces resistance to abiotic stress and biotic diseases. P. indica fungal cell wall extract induces cytoplasmic calcium elevation in host plant roots. Here, we show that cellotriose (CT) is an elicitor-active cell wall moiety released by P. indica into the medium. CT induces a mild defense-like response, including the production of reactive oxygen species, changes in membrane potential, and the expression of genes involved in growth regulation and root development. CT-based cytoplasmic calcium elevation in Arabidopsis (Arabidopsis thaliana) roots does not require the BAK1 coreceptor or the putative Ca(2+) channels TPC1, GLR3.3, GLR2.4, and GLR2.5 and operates synergistically with the elicitor chitin. We identified an ethyl methanesulfonate-induced mutant (cytoplasmiccalcium elevation mutant) impaired in the response to CT and various other cellooligomers (n = 2-7), but not to chitooligomers (n = 4-8), in roots. The mutant contains a single nucleotide exchange in the gene encoding a poly(A) ribonuclease (AtPARN; At1g55870) that degrades the poly(A) tails of specific mRNAs. The wild-type PARN cDNA, expressed under the control of a 35S promoter, complements the mutant phenotype. Our identification of cellotriose as a novel chemical mediator casts light on the complex P. indica-plant mutualistic relationship.},
added-at = {2024-02-15T15:08:22.000+0100},
author = {Johnson, J. M. and Thurich, J. and Petutschnig, E. K. and Altschmied, L. and Meichsner, D. and Sherameti, I. and Dindas, J. and Mrozinska, A. and Paetz, C. and Scholz, S. S. and Furch, A. C. U. and Lipka, V. and Hedrich, R. and Schneider, B. and Svatos, A. and Oelmuller, R.},
biburl = {https://www.bibsonomy.org/bibtex/2add06900f513c2d7375597b04e47d890/jvsi_all},
doi = {10.1104/pp.17.01423},
interhash = {c2348f049a72525e5897dda9edb395e2},
intrahash = {add06900f513c2d7375597b04e47d890},
issn = {1532-2548 (Electronic)
0032-0889 (Print)
0032-0889 (Linking)},
journal = {Plant Physiol},
keywords = {Arabidopsis/genetics/metabolism/*microbiology myOwn},
note = {Johnson, Joy M
Thurich, Johannes
Petutschnig, Elena K
Altschmied, Lothar
Meichsner, Doreen
Sherameti, Irena
Dindas, Julian
Mrozinska, Anna
Paetz, Christian
Scholz, Sandra S
Furch, Alexandra C U
Lipka, Volker
Hedrich, Rainer
Schneider, Bernd
Svatos, Ales
Oelmuller, Ralf
eng
Research Support, Non-U.S. Gov't
2018/01/27
Plant Physiol. 2018 Mar;176(3):2496-2514. doi: 10.1104/pp.17.01423. Epub 2018 Jan 25.},
number = 3,
pages = {2496-2514},
timestamp = {2024-02-15T15:08:22.000+0100},
title = {A Poly(A) Ribonuclease Controls the Cellotriose-Based Interaction between Piriformospora indica and Its Host Arabidopsis},
type = {Journal Article},
url = {https://www.ncbi.nlm.nih.gov/pubmed/29371249},
volume = 176,
year = 2018
}