The genomic clone G-21 which resembles a beta-adrenergic receptor
sequence encodes the 5-HT1A receptor
A. Fargin, J. Raymond, M. Lohse, B. Kobilka, M. Caron, and R. Lefkowitz. Nature, 335 (6188):
358-60(September 1988)Fargin, A Raymond, J R Lohse, M J Kobilka, B K Caron, M G Lefkowitz,
R J Research Support, Non-U.S. Gov't England Nature Nature. 1988
Sep 22;335(6188):358-60..
Abstract
The recent cloning of the complementary DNAs and/or genes for several
receptors linked to guanine nucleotide regulatory proteins including
the adrenergic receptors (alpha 1, alpha 2A, alpha 2B, beta 1, beta
2), several subtypes of the muscarinic cholinergic receptors, and
the visual 'receptor' rhodopsin has revealed considerable similarity
in the primary structure of these proteins. In addition, all of these
proteins contain seven putative transmembrane alpha-helices. We have
previously described a genomic clone, G-21, isolated by cross-hybridization
at reduced stringency with a full length beta 2-adrenergic receptor
probe. This clone contains an intronless gene which, because of its
striking sequence resemblance to the adrenergic receptors, is presumed
to encode a G-protein-coupled receptor. Previous attempts to identify
this putative receptor by expression studies have failed. We now
report that the protein product of the genomic clone, G21, transiently
expressed in monkey kidney cells has all the typical ligand-binding
characteristics of the 5-hydroxytryptamine (5-HT1A) receptor.
Fargin, A Raymond, J R Lohse, M J Kobilka, B K Caron, M G Lefkowitz,
R J Research Support, Non-U.S. Gov't England Nature Nature. 1988
Sep 22;335(6188):358-60.
%0 Journal Article
%1 Fargin1988
%A Fargin, A.
%A Raymond, J. R.
%A Lohse, M. J.
%A Kobilka, B. K.
%A Caron, M. G.
%A Lefkowitz, R. J.
%D 1988
%J Nature
%K Animals Binding Cloning, Cultured GTP-Binding Haplorhini Molecular Protein Proteins/*genetics Serotonin/*genetics Receptor Cell
%N 6188
%P 358-60
%T The genomic clone G-21 which resembles a beta-adrenergic receptor
sequence encodes the 5-HT1A receptor
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3138543
%V 335
%X The recent cloning of the complementary DNAs and/or genes for several
receptors linked to guanine nucleotide regulatory proteins including
the adrenergic receptors (alpha 1, alpha 2A, alpha 2B, beta 1, beta
2), several subtypes of the muscarinic cholinergic receptors, and
the visual 'receptor' rhodopsin has revealed considerable similarity
in the primary structure of these proteins. In addition, all of these
proteins contain seven putative transmembrane alpha-helices. We have
previously described a genomic clone, G-21, isolated by cross-hybridization
at reduced stringency with a full length beta 2-adrenergic receptor
probe. This clone contains an intronless gene which, because of its
striking sequence resemblance to the adrenergic receptors, is presumed
to encode a G-protein-coupled receptor. Previous attempts to identify
this putative receptor by expression studies have failed. We now
report that the protein product of the genomic clone, G21, transiently
expressed in monkey kidney cells has all the typical ligand-binding
characteristics of the 5-hydroxytryptamine (5-HT1A) receptor.
@article{Fargin1988,
abstract = {The recent cloning of the complementary DNAs and/or genes for several
receptors linked to guanine nucleotide regulatory proteins including
the adrenergic receptors (alpha 1, alpha 2A, alpha 2B, beta 1, beta
2), several subtypes of the muscarinic cholinergic receptors, and
the visual 'receptor' rhodopsin has revealed considerable similarity
in the primary structure of these proteins. In addition, all of these
proteins contain seven putative transmembrane alpha-helices. We have
previously described a genomic clone, G-21, isolated by cross-hybridization
at reduced stringency with a full length beta 2-adrenergic receptor
probe. This clone contains an intronless gene which, because of its
striking sequence resemblance to the adrenergic receptors, is presumed
to encode a G-protein-coupled receptor. Previous attempts to identify
this putative receptor by expression studies have failed. We now
report that the protein product of the genomic clone, G21, transiently
expressed in monkey kidney cells has all the typical ligand-binding
characteristics of the 5-hydroxytryptamine (5-HT1A) receptor.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Fargin, A. and Raymond, J. R. and Lohse, M. J. and Kobilka, B. K. and Caron, M. G. and Lefkowitz, R. J.},
biburl = {https://www.bibsonomy.org/bibtex/2b1c611428a34c0f34a6d46ca98e6cbb7/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {04e5c993fff59c5fe5f3e5fffafa6581},
intrahash = {b1c611428a34c0f34a6d46ca98e6cbb7},
issn = {0028-0836 (Print) 0028-0836 (Linking)},
journal = {Nature},
keywords = {Animals Binding Cloning, Cultured GTP-Binding Haplorhini Molecular Protein Proteins/*genetics Serotonin/*genetics Receptor Cell},
month = {Sep 22},
note = {Fargin, A Raymond, J R Lohse, M J Kobilka, B K Caron, M G Lefkowitz,
R J Research Support, Non-U.S. Gov't England Nature Nature. 1988
Sep 22;335(6188):358-60.},
number = 6188,
pages = {358-60},
shorttitle = {The genomic clone G-21 which resembles a beta-adrenergic receptor
sequence encodes the 5-HT1A receptor},
timestamp = {2010-12-14T18:20:48.000+0100},
title = {The genomic clone G-21 which resembles a beta-adrenergic receptor
sequence encodes the 5-HT1A receptor},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=3138543},
volume = 335,
year = 1988
}