The deformability of erythrocytes is a critical determinant of blood
flow in microcirculation. By capturing red blood cells (RBC) with
optical tweezers and dragging them through a viscous fluid we were able
to measure their overall elasticity. We measured, and compared, the RBC
deformability of 15 homozygous patients (HbSS) including five patients
taking hydroxyurea (HU) for at least 6 months (HbSS/HU), 10 subjects
with sickle cell trait (HbAS) and 35 normal controls. Our results showed
that the RBC deformability was significantly lower in haemoglobin S
(HbS) subjects (HbSS and HbAS), except for HbSS/HU cells, whose
deformability was similar to the normal controls. Our data showed that
the laser optical tweezers technique is able to detect differences in
HbS RBC from subjects taking HU, and to differentiate RBC from normal
controls and HbAS, indicating that this is a very sensitive method and
can be applied for detection of drug-response in sickle cell disease.
%0 Journal Article
%1 WOS:000181831300002
%A Brandao, MM
%A Fontes, A
%A Barjas-Castro, ML
%A Barbosa, LC
%A Costa, FF
%A Cesar, CL
%A Saad, STO
%C 35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK
%D 2003
%I BLACKWELL MUNKSGAARD
%J EUROPEAN JOURNAL OF HAEMATOLOGY
%K blood cell cell; deformability} elasticity; hydroxyurea; red sickle tweezers; {optical
%N 4
%P 207-211
%R 10.1034/j.1600-0609.2003.00027.x
%T Optical tweezers for measuring red blood cell elasticity: application to
the study of drug response in sickle cell disease
%V 70
%X The deformability of erythrocytes is a critical determinant of blood
flow in microcirculation. By capturing red blood cells (RBC) with
optical tweezers and dragging them through a viscous fluid we were able
to measure their overall elasticity. We measured, and compared, the RBC
deformability of 15 homozygous patients (HbSS) including five patients
taking hydroxyurea (HU) for at least 6 months (HbSS/HU), 10 subjects
with sickle cell trait (HbAS) and 35 normal controls. Our results showed
that the RBC deformability was significantly lower in haemoglobin S
(HbS) subjects (HbSS and HbAS), except for HbSS/HU cells, whose
deformability was similar to the normal controls. Our data showed that
the laser optical tweezers technique is able to detect differences in
HbS RBC from subjects taking HU, and to differentiate RBC from normal
controls and HbAS, indicating that this is a very sensitive method and
can be applied for detection of drug-response in sickle cell disease.
@article{WOS:000181831300002,
abstract = {The deformability of erythrocytes is a critical determinant of blood
flow in microcirculation. By capturing red blood cells (RBC) with
optical tweezers and dragging them through a viscous fluid we were able
to measure their overall elasticity. We measured, and compared, the RBC
deformability of 15 homozygous patients (HbSS) including five patients
taking hydroxyurea (HU) for at least 6 months (HbSS/HU), 10 subjects
with sickle cell trait (HbAS) and 35 normal controls. Our results showed
that the RBC deformability was significantly lower in haemoglobin S
(HbS) subjects (HbSS and HbAS), except for HbSS/HU cells, whose
deformability was similar to the normal controls. Our data showed that
the laser optical tweezers technique is able to detect differences in
HbS RBC from subjects taking HU, and to differentiate RBC from normal
controls and HbAS, indicating that this is a very sensitive method and
can be applied for detection of drug-response in sickle cell disease.},
added-at = {2022-05-23T20:00:14.000+0200},
address = {35 NORRE SOGADE, PO BOX 2148, DK-1016 COPENHAGEN, DENMARK},
author = {Brandao, MM and Fontes, A and Barjas-Castro, ML and Barbosa, LC and Costa, FF and Cesar, CL and Saad, STO},
biburl = {https://www.bibsonomy.org/bibtex/2bcdb28bbdf4de0b728cc0a13c3339424/ppgfis_ufc_br},
doi = {10.1034/j.1600-0609.2003.00027.x},
interhash = {f3d05b0d5f89f0913fd4b5bb2b0c1d9f},
intrahash = {bcdb28bbdf4de0b728cc0a13c3339424},
issn = {0902-4441},
journal = {EUROPEAN JOURNAL OF HAEMATOLOGY},
keywords = {blood cell cell; deformability} elasticity; hydroxyurea; red sickle tweezers; {optical},
number = 4,
pages = {207-211},
publisher = {BLACKWELL MUNKSGAARD},
pubstate = {published},
timestamp = {2022-05-23T20:00:14.000+0200},
title = {Optical tweezers for measuring red blood cell elasticity: application to
the study of drug response in sickle cell disease},
tppubtype = {article},
volume = 70,
year = 2003
}