OBJECTIVE: The aim of our study was to analyse the single-channel
properties of L-type calcium channels from failing human heart and
to compare them to the respective animal data. Furthermore, we intended
to evaluate the feasibility of future single-channel studies on the
role of calcium channels in the pathophysiology of heart failure.
METHODS: Single L-type calcium channels were recorded in ventricular
myocytes from explanted failing human heart, using the cell-attached
configuration of the patch-clamp technique. RESULTS: One or more
successful registrations of calcium channels could be obtained in
11 of 19 cell isolations. Determination of single-channel conductance
yielded a mean value of 16.6 +/- 1.2 pS (70 mM Ba2+ as the charge
carrier) under control conditions and 23.7 +/- 2.8 pS in presence
of the calcium-channel agonist FPL 64176. The rapid gating process
could be described by a C<-->C<-->O gating scheme. Slow gating analysis
revealed a highly significant clustering of active and non-active
sweeps. CONCLUSION: Single-channel measurements of L-type calcium
channels in human failing ventricle are feasible and reproducible
despite the varying patient characteristics. Their channel properties
are qualitatively comparable to those found in other mammals. Whether
there are quantitative differences due to the underlying heart failure
can be elucidated in further studies.
%0 Journal Article
%1 Hand_1998_445
%A Handrock, R.
%A Schr�der, F.
%A Hirt, S.
%A Haverich, A.
%A Mittmann, C.
%A Herzig, S.
%D 1998
%J Cardiovasc. Res.
%K 8-Bromo 9614499 AMP, Adenosine Adult, Aged, Agonists, Atria, Barium, Calcineurin, Calcium Calcium, Cardiomyopathies, Cardiomyopathy, Cell Cells, Channel Channels, Comparative Congestive, Cultured, Cyclic Cyclosporine, Dilated, Factors, Failure, Female, Gating, Gov't, Heart Heart, Humans, Ion Ischemia, Kinetics, L-Type, Line, Male, Membrane Middle Monophosphate, Muscle Myocardia, Non-U.S. P.H.S., Patch-Clamp Potentials, Probability, Proteins, Pyrroles, Recombinant Reference Research Study, Support, Techniques, Time U.S. Values, Ventricles, l
%N 2
%P 445--455
%T Single-channel properties of L-type calcium channels from failing
human ventricle.
%U http://dx.doi.org/10.1016/S0008-6363(97)00257-5
%V 37
%X OBJECTIVE: The aim of our study was to analyse the single-channel
properties of L-type calcium channels from failing human heart and
to compare them to the respective animal data. Furthermore, we intended
to evaluate the feasibility of future single-channel studies on the
role of calcium channels in the pathophysiology of heart failure.
METHODS: Single L-type calcium channels were recorded in ventricular
myocytes from explanted failing human heart, using the cell-attached
configuration of the patch-clamp technique. RESULTS: One or more
successful registrations of calcium channels could be obtained in
11 of 19 cell isolations. Determination of single-channel conductance
yielded a mean value of 16.6 +/- 1.2 pS (70 mM Ba2+ as the charge
carrier) under control conditions and 23.7 +/- 2.8 pS in presence
of the calcium-channel agonist FPL 64176. The rapid gating process
could be described by a C<-->C<-->O gating scheme. Slow gating analysis
revealed a highly significant clustering of active and non-active
sweeps. CONCLUSION: Single-channel measurements of L-type calcium
channels in human failing ventricle are feasible and reproducible
despite the varying patient characteristics. Their channel properties
are qualitatively comparable to those found in other mammals. Whether
there are quantitative differences due to the underlying heart failure
can be elucidated in further studies.
@article{Hand_1998_445,
abstract = {O{BJECTIVE}: The aim of our study was to analyse the single-channel
properties of L-type calcium channels from failing human heart and
to compare them to the respective animal data. Furthermore, we intended
to evaluate the feasibility of future single-channel studies on the
role of calcium channels in the pathophysiology of heart failure.
METHODS: Single L-type calcium channels were recorded in ventricular
myocytes from explanted failing human heart, using the cell-attached
configuration of the patch-clamp technique. RESULTS: One or more
successful registrations of calcium channels could be obtained in
11 of 19 cell isolations. Determination of single-channel conductance
yielded a mean value of 16.6 +/- 1.2 pS (70 mM Ba2+ as the charge
carrier) under control conditions and 23.7 +/- 2.8 pS in presence
of the calcium-channel agonist {FPL} 64176. The rapid gating process
could be described by a C<-->C<-->O gating scheme. Slow gating analysis
revealed a highly significant clustering of active and non-active
sweeps. CONCLUSION: Single-channel measurements of L-type calcium
channels in human failing ventricle are feasible and reproducible
despite the varying patient characteristics. Their channel properties
are qualitatively comparable to those found in other mammals. Whether
there are quantitative differences due to the underlying heart failure
can be elucidated in further studies.},
added-at = {2009-06-03T11:20:58.000+0200},
author = {Handrock, R. and Schr�der, F. and Hirt, S. and Haverich, A. and Mittmann, C. and Herzig, S.},
biburl = {https://www.bibsonomy.org/bibtex/2d362c17e55ce4a2c8b69e85d1e11ae9e/hake},
description = {The whole bibliography file I use.},
file = {Hand_1998_445.pdf:Hand_1998_445.pdf:PDF},
interhash = {78f6d5fa290e9bb583cfa320a2838fd0},
intrahash = {d362c17e55ce4a2c8b69e85d1e11ae9e},
journal = {Cardiovasc. Res.},
key = 159,
keywords = {8-Bromo 9614499 AMP, Adenosine Adult, Aged, Agonists, Atria, Barium, Calcineurin, Calcium Calcium, Cardiomyopathies, Cardiomyopathy, Cell Cells, Channel Channels, Comparative Congestive, Cultured, Cyclic Cyclosporine, Dilated, Factors, Failure, Female, Gating, Gov't, Heart Heart, Humans, Ion Ischemia, Kinetics, L-Type, Line, Male, Membrane Middle Monophosphate, Muscle Myocardia, Non-U.S. P.H.S., Patch-Clamp Potentials, Probability, Proteins, Pyrroles, Recombinant Reference Research Study, Support, Techniques, Time U.S. Values, Ventricles, l},
month = Feb,
number = 2,
pages = {445--455},
pii = {S0008636397002575},
pmid = {9614499},
timestamp = {2009-06-03T11:21:13.000+0200},
title = {Single-channel properties of L-type calcium channels from failing
human ventricle.},
url = {http://dx.doi.org/10.1016/S0008-6363(97)00257-5},
volume = 37,
year = 1998
}