We report here that the surface topography of colloidal mesoporous
silica nanoparticles (MSNs) plays a key role on their
bionano-interactions by driving the adsorption of biomolecules on the
nanoparticle through a matching mechanism between the surface cavities
characteristics and the biomolecules stereochemistry. This conclusion
was drawn by analyzing the biophysicochemical properties of colloidal
MSNs in the presence of single biomolecules, such as alginate or bovine
serum albumin (BSA), as well as dispersed in a complex biofluid, such as
human blood plasma. When dispersed in phosphate buffered saline media
containing alginate or BSA, monodisperse spherical MSNs interact with
linear biopolymers such as alginate and with a globular protein such as
bovine serum albumin (BSA) independently of the surface charge sign
(i.e. positive or negative), thus leading to a decrease in the surface
energy and to the colloidal stabilization of these nanoparticles. In
contrast, silica nanoparticles with irregular surface topographies are
not colloidally stabilized in the presence of alginate but they are
electrosterically stabilized by BSA through a sorption mechanism that
implies reversible conformation changes of the protein, as evidenced by
circular dichroism (CD). The match between the biomolecule size and
stereochemistry with the nanoparticle surface cavities characteristics
reflects on the nanoparticle surface area that is accessible for each
biomolecule to interact and stabilize any non-rigid nanoparticles. On
the other hand, in contact with variety of biomolecules such as those
present in blood plasma (55%), MSNs are colloidally stabilized
regardless of the topography and surface charge, although the identity
of the protein corona responsible for this stabilization is influenced
by the surface topography and surface charge. Therefore, the biofluid in
which nanoparticles are introduced plays an important role on their
physicochemical behavior synergistically with their inherent
characteristics (e.g., surface topography).
%0 Journal Article
%1 WOS:000332922900050
%A Paula, Amauri J
%A Silveira, Camila P
%A Martinez, Diego Stefani T
%A Filho, Antonio G Souza
%A Romero, Fabian V
%A Fonseca, Leandro C
%A Tasic, Ljubica
%A Alves, Oswaldo L
%A Duran, Nelson
%C 1155 16TH ST, NW, WASHINGTON, DC 20036 USA
%D 2014
%I AMER CHEMICAL SOC
%J ACS APPLIED MATERIALS & INTERFACES
%K albumin; alginate; biomolecules blood bovine colloidal corona; human interaction; nanoparticles; plasma} protein serum silica; {mesoporous
%N 5
%P 3437-3447
%R 10.1021/am405594q
%T Topography-driven bionano-interactions on colloidal silica nanoparticles
%V 6
%X We report here that the surface topography of colloidal mesoporous
silica nanoparticles (MSNs) plays a key role on their
bionano-interactions by driving the adsorption of biomolecules on the
nanoparticle through a matching mechanism between the surface cavities
characteristics and the biomolecules stereochemistry. This conclusion
was drawn by analyzing the biophysicochemical properties of colloidal
MSNs in the presence of single biomolecules, such as alginate or bovine
serum albumin (BSA), as well as dispersed in a complex biofluid, such as
human blood plasma. When dispersed in phosphate buffered saline media
containing alginate or BSA, monodisperse spherical MSNs interact with
linear biopolymers such as alginate and with a globular protein such as
bovine serum albumin (BSA) independently of the surface charge sign
(i.e. positive or negative), thus leading to a decrease in the surface
energy and to the colloidal stabilization of these nanoparticles. In
contrast, silica nanoparticles with irregular surface topographies are
not colloidally stabilized in the presence of alginate but they are
electrosterically stabilized by BSA through a sorption mechanism that
implies reversible conformation changes of the protein, as evidenced by
circular dichroism (CD). The match between the biomolecule size and
stereochemistry with the nanoparticle surface cavities characteristics
reflects on the nanoparticle surface area that is accessible for each
biomolecule to interact and stabilize any non-rigid nanoparticles. On
the other hand, in contact with variety of biomolecules such as those
present in blood plasma (55%), MSNs are colloidally stabilized
regardless of the topography and surface charge, although the identity
of the protein corona responsible for this stabilization is influenced
by the surface topography and surface charge. Therefore, the biofluid in
which nanoparticles are introduced plays an important role on their
physicochemical behavior synergistically with their inherent
characteristics (e.g., surface topography).
@article{WOS:000332922900050,
abstract = {We report here that the surface topography of colloidal mesoporous
silica nanoparticles (MSNs) plays a key role on their
bionano-interactions by driving the adsorption of biomolecules on the
nanoparticle through a matching mechanism between the surface cavities
characteristics and the biomolecules stereochemistry. This conclusion
was drawn by analyzing the biophysicochemical properties of colloidal
MSNs in the presence of single biomolecules, such as alginate or bovine
serum albumin (BSA), as well as dispersed in a complex biofluid, such as
human blood plasma. When dispersed in phosphate buffered saline media
containing alginate or BSA, monodisperse spherical MSNs interact with
linear biopolymers such as alginate and with a globular protein such as
bovine serum albumin (BSA) independently of the surface charge sign
(i.e. positive or negative), thus leading to a decrease in the surface
energy and to the colloidal stabilization of these nanoparticles. In
contrast, silica nanoparticles with irregular surface topographies are
not colloidally stabilized in the presence of alginate but they are
electrosterically stabilized by BSA through a sorption mechanism that
implies reversible conformation changes of the protein, as evidenced by
circular dichroism (CD). The match between the biomolecule size and
stereochemistry with the nanoparticle surface cavities characteristics
reflects on the nanoparticle surface area that is accessible for each
biomolecule to interact and stabilize any non-rigid nanoparticles. On
the other hand, in contact with variety of biomolecules such as those
present in blood plasma (55%), MSNs are colloidally stabilized
regardless of the topography and surface charge, although the identity
of the protein corona responsible for this stabilization is influenced
by the surface topography and surface charge. Therefore, the biofluid in
which nanoparticles are introduced plays an important role on their
physicochemical behavior synergistically with their inherent
characteristics (e.g., surface topography).},
added-at = {2022-05-23T20:00:14.000+0200},
address = {1155 16TH ST, NW, WASHINGTON, DC 20036 USA},
author = {Paula, Amauri J and Silveira, Camila P and Martinez, Diego Stefani T and Filho, Antonio G Souza and Romero, Fabian V and Fonseca, Leandro C and Tasic, Ljubica and Alves, Oswaldo L and Duran, Nelson},
biburl = {https://www.bibsonomy.org/bibtex/2dae81cb57f99a0b0546a529ec61cf371/ppgfis_ufc_br},
doi = {10.1021/am405594q},
interhash = {13c4fd7905de1ebdb90824c8820054c1},
intrahash = {dae81cb57f99a0b0546a529ec61cf371},
issn = {1944-8244},
journal = {ACS APPLIED MATERIALS & INTERFACES},
keywords = {albumin; alginate; biomolecules blood bovine colloidal corona; human interaction; nanoparticles; plasma} protein serum silica; {mesoporous},
number = 5,
pages = {3437-3447},
publisher = {AMER CHEMICAL SOC},
pubstate = {published},
timestamp = {2022-05-23T20:00:14.000+0200},
title = {Topography-driven bionano-interactions on colloidal silica nanoparticles},
tppubtype = {article},
volume = 6,
year = 2014
}