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Preparation and characterisation of oligosaccharides produced by nitrous acid depolymerisation of chitosans

, , , and . Carbohydr. Res. 333 (2): 137 -- 144 (July 2001)

Abstract

Two chitosans with widely different chemical composition (fraction of N-acetylated units (F-A)<0.001 and F-A= 0.59), were degraded by nitrous acid, to obtain the reactive 2,5-anhydro-D-mannose- (M-) unit at the new reducing end. The fully N-acetylated and fully N-deacetylated oligomers were separated by size-exclusion chromatography. Both the chemical structure and purity were studied by one- and two-dimensional H-1 and C-13 NMR methods. The fully N-acetylated oligomers were found to be stable, whereas the N-deacetylated oligomers reacted intermolecularly by a Schiff base reaction between the 2-amino group on the N-deacetylated units and the M-units, facilitating the cleavage of the glycosidic bond next to the M-unit and the formation of 5-hydroxymethylfurfural (HMF). (C) 2001 Elsevier Science Ltd. All rights reserved.

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Tommeraas2001
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