%0 Journal Article %1 Lu:2004p4181 %A Lu, HT %A Jiang, Y %A Chen, F %D 2004 %J Chromatographia %K Alpha-Tocopherol, And Cholesterol, Chromatography, Column Crystallization, Extraction Fraction, Fractional From Gas-Chromatography, Liquid Natural Oil Olive Pharmaceutical Rat, Sources, Squalene Sterols, %N 5-6 %P 367--371 %R 10.1365/s10337-003-0176-6 %T Determination of squalene using high-performance liquid chromatography with diode array detection %U http://springerlink.metapress.com/content/j9qleb7muplx2lgj/ %V 59 %X A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C-18 column (150 x 3.9 mm, 5 mum) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 10040000 mugL(-1) and the detection limit was 40 mugL(-1). The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.

@article{Lu:2004p4181, abstract = {A high-performance liquid chromatographic method with photodiode array detection has been developed for the determination of squalene. After treated by extraction and fractional crystallization, squalene was analyzed on a C-18 column (150 x 3.9 mm, 5 mum) with acetonitrile as mobile phase. Excellent linearity of the calibration curve was observed in the range of 10040000 mugL(-1) and the detection limit was 40 mugL(-1). The recoveries were from 89.6% to 100.5% and the relative standard deviations were from 0.5% to 1.4%. The method was successfully applied to the determination of squalene in squalene capsules, olive oil, algal lipids and algal cells.}, added-at = {2010-06-22T19:38:37.000+0200}, affiliation = {Hong Kong Baptist Univ, Dept Biol, Kowloon Tong, Hong Kong, Peoples R China}, author = {Lu, HT and Jiang, Y and Chen, F}, biburl = {https://www.bibsonomy.org/bibtex/2f0d3787cc00ae39fef69f34a7fb030de/gsmith}, date-added = {2010-06-11 13:54:43 -0400}, date-modified = {2010-06-11 13:54:50 -0400}, doi = {10.1365/s10337-003-0176-6}, interhash = {a19514facdffeca94c25a05d9ea65af2}, intrahash = {f0d3787cc00ae39fef69f34a7fb030de}, journal = {Chromatographia}, keywords = {Alpha-Tocopherol, And Cholesterol, Chromatography, Column Crystallization, Extraction Fraction, Fractional From Gas-Chromatography, Liquid Natural Oil Olive Pharmaceutical Rat, Sources, Squalene Sterols,}, language = {English}, local-url = {file://localhost/Users/geoffreysmith/Documents/Papers/Chromatographia/2004/Chromatographia,%2059,%20367-371%202004.pdf}, month = Jan, number = {5-6}, pages = {367--371}, pmid = {000224099900015}, rating = {0}, timestamp = {2010-06-22T19:39:31.000+0200}, title = {Determination of squalene using high-performance liquid chromatography with diode array detection}, uri = {papers://E88B624E-D406-46FF-9D95-BB9C1AAE3FDC/Paper/p4181}, url = {http://springerlink.metapress.com/content/j9qleb7muplx2lgj/}, volume = 59, year = 2004 }