Immunofluorescent imaging of beta 1- and beta 2-adrenergic receptors
in rat kidney
V. Boivin, R. Jahns, S. Gambaryan, W. Ness, F. Boege, and M. Lohse. Kidney Int, 59 (2):
515-31(February 2001)Boivin, V Jahns, R Gambaryan, S Ness, W Boege, F Lohse, M J Research
Support, Non-U.S. Gov't United States Kidney international Kidney
Int. 2001 Feb;59(2):515-31..
Abstract
BACKGROUND: beta-Adrenergic receptors (beta-ARs) are known to participate
in the regulation of glomerular filtration, NaCl reabsorption, acid-base
balance, and renin secretion; however, the precise histologic localization
of beta-AR at putative signaling sites involved in these processes
remains an open issue. METHODS: We used a set of subtype-specific
rabbit antibodies to visualize beta(1)- and beta(2)-AR in rat kidney
by immunohistochemistry and specified cells and segments of the nephron
thought to be regulated by catecholamines. In addition, the relative
proportion of beta-AR subtypes in cortical and medullary portions
of rat kidney was determined by Western blotting and by competing
(125)I-cyanopindolol binding with the beta(1)- or beta(2)-selective
antagonists bisoprolol or ICI 118,551, respectively. RESULTS: Immunoreactivity
for beta(1)-AR was found in mesangial cells, juxtaglomerular granular
cells, the macula densa epithelium, proximal and distal tubular segments,
and acid-secreting type A intercalated cells of the cortical and
medullary collecting ducts. Immunoreactivity for beta(2)-AR was predominantly
localized in the apical and subapical compartment of proximal and,
to a lesser extent, distal tubular epithelia (suggesting interactions
with luminal fluid catecholamines). Both subtypes were dense in the
membranes of smooth muscle cells from renal arteries. Concordant
data were obtained by radioligand binding and immunoblotting of membranes
prepared from cortical and medullary portions of the kidney. CONCLUSION:
Our data provide an immunohistochemical basis for the cellular targets
of beta-adrenergic regulation of renal function. Moreover, they could
help to devise therapeutic strategies directed at renal beta-ARs.
Boivin, V Jahns, R Gambaryan, S Ness, W Boege, F Lohse, M J Research
Support, Non-U.S. Gov't United States Kidney international Kidney
Int. 2001 Feb;59(2):515-31.
%0 Journal Article
%1 Boivin2001
%A Boivin, V.
%A Jahns, R.
%A Gambaryan, S.
%A Ness, W.
%A Boege, F.
%A Lohse, M. J.
%D 2001
%J Kidney Int
%K Animals Antibody Assay Distribution Fluorescent Immunoblotting Kidney/*metabolism Male Radioligand Rats Sprague-Dawley Technique Tissue beta/*metabolism/physiology Receptor Adrenergic
%N 2
%P 515-31
%T Immunofluorescent imaging of beta 1- and beta 2-adrenergic receptors
in rat kidney
%U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11168934
%V 59
%X BACKGROUND: beta-Adrenergic receptors (beta-ARs) are known to participate
in the regulation of glomerular filtration, NaCl reabsorption, acid-base
balance, and renin secretion; however, the precise histologic localization
of beta-AR at putative signaling sites involved in these processes
remains an open issue. METHODS: We used a set of subtype-specific
rabbit antibodies to visualize beta(1)- and beta(2)-AR in rat kidney
by immunohistochemistry and specified cells and segments of the nephron
thought to be regulated by catecholamines. In addition, the relative
proportion of beta-AR subtypes in cortical and medullary portions
of rat kidney was determined by Western blotting and by competing
(125)I-cyanopindolol binding with the beta(1)- or beta(2)-selective
antagonists bisoprolol or ICI 118,551, respectively. RESULTS: Immunoreactivity
for beta(1)-AR was found in mesangial cells, juxtaglomerular granular
cells, the macula densa epithelium, proximal and distal tubular segments,
and acid-secreting type A intercalated cells of the cortical and
medullary collecting ducts. Immunoreactivity for beta(2)-AR was predominantly
localized in the apical and subapical compartment of proximal and,
to a lesser extent, distal tubular epithelia (suggesting interactions
with luminal fluid catecholamines). Both subtypes were dense in the
membranes of smooth muscle cells from renal arteries. Concordant
data were obtained by radioligand binding and immunoblotting of membranes
prepared from cortical and medullary portions of the kidney. CONCLUSION:
Our data provide an immunohistochemical basis for the cellular targets
of beta-adrenergic regulation of renal function. Moreover, they could
help to devise therapeutic strategies directed at renal beta-ARs.
@article{Boivin2001,
abstract = {BACKGROUND: beta-Adrenergic receptors (beta-ARs) are known to participate
in the regulation of glomerular filtration, NaCl reabsorption, acid-base
balance, and renin secretion; however, the precise histologic localization
of beta-AR at putative signaling sites involved in these processes
remains an open issue. METHODS: We used a set of subtype-specific
rabbit antibodies to visualize beta(1)- and beta(2)-AR in rat kidney
by immunohistochemistry and specified cells and segments of the nephron
thought to be regulated by catecholamines. In addition, the relative
proportion of beta-AR subtypes in cortical and medullary portions
of rat kidney was determined by Western blotting and by competing
[(125)I]-cyanopindolol binding with the beta(1)- or beta(2)-selective
antagonists bisoprolol or ICI 118,551, respectively. RESULTS: Immunoreactivity
for beta(1)-AR was found in mesangial cells, juxtaglomerular granular
cells, the macula densa epithelium, proximal and distal tubular segments,
and acid-secreting type A intercalated cells of the cortical and
medullary collecting ducts. Immunoreactivity for beta(2)-AR was predominantly
localized in the apical and subapical compartment of proximal and,
to a lesser extent, distal tubular epithelia (suggesting interactions
with luminal fluid catecholamines). Both subtypes were dense in the
membranes of smooth muscle cells from renal arteries. Concordant
data were obtained by radioligand binding and immunoblotting of membranes
prepared from cortical and medullary portions of the kidney. CONCLUSION:
Our data provide an immunohistochemical basis for the cellular targets
of beta-adrenergic regulation of renal function. Moreover, they could
help to devise therapeutic strategies directed at renal beta-ARs.},
added-at = {2010-12-14T18:12:02.000+0100},
author = {Boivin, V. and Jahns, R. and Gambaryan, S. and Ness, W. and Boege, F. and Lohse, M. J.},
biburl = {https://www.bibsonomy.org/bibtex/2f49a80d45b16544bc2865b96b13d2d69/pharmawuerz},
endnotereftype = {Journal Article},
interhash = {f0f579ce4a7be2f21bdd7f082d31b876},
intrahash = {f49a80d45b16544bc2865b96b13d2d69},
issn = {0085-2538 (Print) 0085-2538 (Linking)},
journal = {Kidney Int},
keywords = {Animals Antibody Assay Distribution Fluorescent Immunoblotting Kidney/*metabolism Male Radioligand Rats Sprague-Dawley Technique Tissue beta/*metabolism/physiology Receptor Adrenergic},
month = Feb,
note = {Boivin, V Jahns, R Gambaryan, S Ness, W Boege, F Lohse, M J Research
Support, Non-U.S. Gov't United States Kidney international Kidney
Int. 2001 Feb;59(2):515-31.},
number = 2,
pages = {515-31},
shorttitle = {Immunofluorescent imaging of beta 1- and beta 2-adrenergic receptors
in rat kidney},
timestamp = {2010-12-14T18:22:56.000+0100},
title = {Immunofluorescent imaging of beta 1- and beta 2-adrenergic receptors
in rat kidney},
url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=11168934},
volume = 59,
year = 2001
}