%0 Journal Article %1 citeulike:489605 %A Taddei-Peters, W. C. %A Butman, B. T. %A Jones, G. R. %A Venetta, T. M. %A Macomber, P. F. %A Ransom, J. H. %C Organon Teknika/Biotechnology Research Institute, Rockville, MD 20850. %D 1993 %J Clinical Chemistry %K apoa elisa apob %N 7 %P 1382--1389 %T Quantification of lipoprotein(a) particles containing various apolipoprotein(a) isoforms by a monoclonal anti-apo(a) capture antibody and a polyclonal anti-apolipoprotein B detection antibody sandwich enzyme immunoassay. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=7687203 %V 39 %X A quantitative sandwich ELISA for lipoprotein(a) Lp(a), utilizing a monoclonal capture antibody that recognizes human and rhesus monkey apolipoprotein(a) apo(a) isoforms in combination with a polyclonal anti-apolipoprotein B-peroxidase conjugate was developed. This assay generates a linear calibration curve from 31.2 to 1000 mg/L, is highly reproducible (intra- and interassay CV of < 5% and < or = 12%, respectively), and shows no interference from plasminogen (1 g/L), low-density lipoprotein (6.00 g/L), triglycerides (27.00 g/L from chylomicrons and 10.00 g/L from very-low-density lipoprotein), hemoglobin (5 g/L), or bilirubin (30 mg/L). This assay format quantifies the concentration of Lp(a) on an equal molar basis regardless of apo(a) isoform. In contrast, a commercially available ELISA Macra Lp(a) method with a monoclonal anti-apo(a) capture antibody and a polyclonal anti-apo(a) conjugate was found to underestimate the Lp(a) concentrations of individuals with lower-M(r) apo(a) isoforms--whether quantifying the Lp(a) in plasma or the purified lipoprotein. This demonstrates the importance of assay format selection in quantifying Lp(a).

@article{citeulike:489605, abstract = {A quantitative sandwich ELISA for lipoprotein(a) [Lp(a)], utilizing a monoclonal capture antibody that recognizes human and rhesus monkey apolipoprotein(a) [apo(a)] isoforms in combination with a polyclonal anti-apolipoprotein B-peroxidase conjugate was developed. This assay generates a linear calibration curve from 31.2 to 1000 mg/L, is highly reproducible (intra- and interassay CV of < 5% and < or = 12%, respectively), and shows no interference from plasminogen (1 g/L), low-density lipoprotein (6.00 g/L), triglycerides (27.00 g/L from chylomicrons and 10.00 g/L from very-low-density lipoprotein), hemoglobin (5 g/L), or bilirubin (30 mg/L). This assay format quantifies the concentration of Lp(a) on an equal molar basis regardless of apo(a) isoform. In contrast, a commercially available ELISA [Macra Lp(a)] method with a monoclonal anti-apo(a) capture antibody and a polyclonal anti-apo(a) conjugate was found to underestimate the Lp(a) concentrations of individuals with lower-M(r) apo(a) isoforms--whether quantifying the Lp(a) in plasma or the purified lipoprotein. This demonstrates the importance of assay format selection in quantifying Lp(a).}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Organon Teknika/Biotechnology Research Institute, Rockville, MD 20850.}, author = {Taddei-Peters, W. C. and Butman, B. T. and Jones, G. R. and Venetta, T. M. and Macomber, P. F. and Ransom, J. H.}, biburl = {https://www.bibsonomy.org/bibtex/2fcd536e1fb36a885f8e23eb2166e1bed/biblio24}, citeulike-article-id = {489605}, interhash = {2c987d7a4cf6b61fee62b150b95ba31d}, intrahash = {fcd536e1fb36a885f8e23eb2166e1bed}, issn = {0009-9147}, journal = {Clinical Chemistry}, keywords = {apoa elisa apob}, month = {July}, number = 7, pages = {1382--1389}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {Quantification of lipoprotein(a) particles containing various apolipoprotein(a) isoforms by a monoclonal anti-apo(a) capture antibody and a polyclonal anti-apolipoprotein B detection antibody sandwich enzyme immunoassay.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=7687203}, volume = 39, year = 1993 }