%0 Journal Article %1 chelico2006apobec3g %A Chelico, Linda %A Pham, Phuong %A Calabrese, Peter %A Goodman, Myron F %D 2006 %J Nat Struct Mol Biol %K APOBEC HIV context-dependent-mutation hypermutability mutation_spectrum %N 5 %P 392--399 %T APOBEC3G DNA deaminase acts processively 3' $\to$ 5' on single-stranded DNA %U http://dx.doi.org/10.1038/nsmb1086 %V 13 %X Akin to a ‘Trojan horse,’ APOBEC3G DNA deaminase is encapsulated by the HIV virion. APOBEC3G facilitates restriction of HIV-1 infection in T cells by deaminating cytosines in nascent minus-strand complementary DNA. Here, we investigate the biochemical basis for C-U targeting. We observe that APOBEC3G binds randomly to single-stranded DNA, then jumps and slides processively to deaminate target motifs. When confronting partially double-stranded DNA, to which APOBEC3G cannot bind, sliding is lost but jumping is retained. APOBEC3G shows catalytic orientational specificity such that deamination occurs predominantly 3¢-5¢ without requiring hydrolysis of a nucleotide cofactor. Our data suggest that the G-A mutational gradient generated in viral genomic DNA in vivo could result from an intrinsic processive directional attack by APOBEC3G on single- stranded cDNA

@article{chelico2006apobec3g, abstract = {Akin to a ‘Trojan horse,’ APOBEC3G DNA deaminase is encapsulated by the HIV virion. APOBEC3G facilitates restriction of HIV-1 infection in T cells by deaminating cytosines in nascent minus-strand complementary DNA. Here, we investigate the biochemical basis for C-U targeting. We observe that APOBEC3G binds randomly to single-stranded DNA, then jumps and slides processively to deaminate target motifs. When confronting partially double-stranded DNA, to which APOBEC3G cannot bind, sliding is lost but jumping is retained. APOBEC3G shows catalytic orientational specificity such that deamination occurs predominantly 3¢-5¢ without requiring hydrolysis of a nucleotide cofactor. Our data suggest that the G-A mutational gradient generated in viral genomic DNA in vivo could result from an intrinsic processive directional attack by APOBEC3G on single- stranded cDNA}, added-at = {2014-07-02T00:28:15.000+0200}, author = {Chelico, Linda and Pham, Phuong and Calabrese, Peter and Goodman, Myron F}, biburl = {https://www.bibsonomy.org/bibtex/204fd8c9a3555d6976f9538e8a9e0bdf0/peter.ralph}, comment = {10.1038/nsmb1086}, interhash = {3328dc71fae10e3b8e1bc2d0bc734a9e}, intrahash = {04fd8c9a3555d6976f9538e8a9e0bdf0}, issn = {15459993}, journal = {Nat Struct Mol Biol}, keywords = {APOBEC HIV context-dependent-mutation hypermutability mutation_spectrum}, month = may, number = 5, pages = {392--399}, timestamp = {2014-07-02T00:32:34.000+0200}, title = {{APOBEC3G} {DNA} deaminase acts processively 3' $\to$ 5' on single-stranded {DNA}}, url = {http://dx.doi.org/10.1038/nsmb1086}, volume = 13, year = 2006 }