%0 Journal Article %1 de_voer_simultaneous_2009 %A de Voer, Richarda M %A Schepp, Rutger M %A Versteegh, Florens G A %A van der Klis, Fiona R M %A Berbers, Guy A M %D 2009 %J Clinical and Vaccine Immunology: CVI %K A, Antibodies, Antigens, Bacterial, C, Cross Fluorescence, G, Haemophilus Humans, Immunoassay, Immunoglobulin Microspheres, Neisseria Polysaccharides, Reactions, Sensitivity Serogroup Specificity W-135, Y, and b, influenzae meningitidis, type %N 3 %P 433--436 %R 10.1128/CVI.00364-08 %T Simultaneous detection of Haemophilus influenzae type b polysaccharide-specific antibodies and Neisseria meningitidis serogroup A, C, Y, and W-135 polysaccharide-specific antibodies in a fluorescent-bead-based multiplex immunoassay %U http://www.ncbi.nlm.nih.gov/pubmed/19129470 %V 16 %X We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay (MIA) to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide (HibPS). The monoplex HibPS assay was compared to a HibPS-specific competitive enzyme-linked immunosorbent assay and showed a good correlation (R=0.96). Furthermore, no cross-reactivity between HibPS and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib MIA is a useful tool to investigate serological responses toward different childhood PS vaccines.

@article{de_voer_simultaneous_2009, abstract = {We expanded the meningococcal serogroup A, C, Y, and W-135 multiplex immunoassay {(MIA)} to simultaneously detect immunoglobulin type G antibodies directed toward Haemophilus influenzae type b polysaccharide {(HibPS).} The monoplex {HibPS} assay was compared to a {HibPS-specific} competitive enzyme-linked immunosorbent assay and showed a good correlation {(R=0.96).} Furthermore, no cross-reactivity between {HibPS} and the four meningococcal serogroups was detected. This pentaplex meningococcal Hib {MIA} is a useful tool to investigate serological responses toward different childhood {PS} vaccines.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {de Voer, Richarda M and Schepp, Rutger M and Versteegh, Florens G A and van der Klis, Fiona R M and Berbers, Guy A M}, biburl = {https://www.bibsonomy.org/bibtex/2e98361d0685ede23b8ff0e44d5804f22/jelias}, doi = {10.1128/CVI.00364-08}, interhash = {35b88dbd67353fc3958c2b47306d9d86}, intrahash = {e98361d0685ede23b8ff0e44d5804f22}, issn = {{1556-679X}}, journal = {Clinical and Vaccine Immunology: {CVI}}, keywords = {A, Antibodies, Antigens, Bacterial, C, Cross Fluorescence, G, Haemophilus Humans, Immunoassay, Immunoglobulin Microspheres, Neisseria Polysaccharides, Reactions, Sensitivity Serogroup Specificity W-135, Y, and b, influenzae meningitidis, type}, month = mar, note = {{PMID:} 19129470}, number = 3, pages = {433--436}, timestamp = {2011-03-11T10:06:41.000+0100}, title = {Simultaneous detection of Haemophilus influenzae type b polysaccharide-specific antibodies and Neisseria meningitidis serogroup A, C, Y, and W-135 polysaccharide-specific antibodies in a fluorescent-bead-based multiplex immunoassay}, url = {http://www.ncbi.nlm.nih.gov/pubmed/19129470}, volume = 16, year = 2009 }