%0 Generic %1 mizuno2013combined %A Mizuno, K %A Narita, E %A Yamada, M %A Shinohara, N %A Miyakoshi, J %D 2013 %K DNA ELISA UV-C WST assay cell dimer method pigmentsum pyrimidine survival synthesis type variant xeroderma %T The combined effects of ELF magnetic field exposure and ultraviolet irradiation %X The potential health risks of extremely low frequency (ELF) magnetic fields are a public concern. Investigations of the potential relationship(s) with human health are very important, and many studies have been sought to elucidate the biological effects of ELF magnetic fields. Once, most investigations have evaluated the effects of ELF magnetic field alone. In 2007, the World Health Organization (WHO) with their Task Group concluded that ELF magnetic field alone have no carcinogenic potential and recommended that further investigations include the evaluation of the combined carcinogenic effects of ELF magnetic field in vitro. Ultraviolet (UV) irradiation can affect many kinds of cellular targets, including nucleic acids, proteins and lipids. DNA is the most important target of UV irradiation. In this study, we evaluated the combined effects of ELF magnetic field exposure on cell survival and DNA damage induced by UV irradiation The ELF magnetic field exposure was conducted with a Helmholtz coil-based exposure system, which was designed to generate a sinusoidal magnetic field at 5 millitesla (mT) and 50/60 Hz. UV irradiation was performed with specially designed apparatus with five UV-Clamps (mainly 253.7 nm) at room temperature. Human embryo lung-derived SV40 virus transformed WI38VA13 subclone 2RA cells were cultured in Eagle's minimum essential medium with non-essential amino acids, lmM sodium pyruvate and 10% fetal bovine serum (FBS). Human xeroderma pigmentosum (valiant type )-derived XP2SA cells were cultured in Dulbecco's modified Eagle's medium with 10% FBS. The cell survival, DNA synthesis and DNA damage were assessed by WST assay, IdU assay and quantification of cyclobutane pyrimidine dimer formation with ELISA method, respectively. WI38VA13 subclone 2RA cells were exposed to magnetic field at 5 mT and 50/60 Hz for 24 hours before/after UV irradiation at 0, 2, 4, 6 and 8 J/m2 • XP2SA cells were analyzed as described for the WI38V A 13 subclone 2RA cells, except that UV irradiation was performed at 0.5, 1, 1.5 and 2 J/m2 for cell survival assay and DNA synthesis assay, and 1, 2, 3 and 4 J/m2 for DNA damage assay. No significant differences were observed in cell survival between ELF magnetic field and sham exposures. Similarly, DNA damages induced by UV irradiation were not changed significantly by ELF magnetic field exposure. Our results suggest that ELF magnetic field exposure at 5 mT does not affect cell survival and DNA damage induced by UV irradiation.

@conference{mizuno2013combined, abstract = {The potential health risks of extremely low frequency (ELF) magnetic fields are a public concern. Investigations of the potential relationship(s) with human health are very important, and many studies have been sought to elucidate the biological effects of ELF magnetic fields. Once, most investigations have evaluated the effects of ELF magnetic field alone. In 2007, the World Health Organization (WHO) with their Task Group concluded that ELF magnetic field alone have no carcinogenic potential and recommended that further investigations include the evaluation of the combined carcinogenic effects of ELF magnetic field in vitro. Ultraviolet (UV) irradiation can affect many kinds of cellular targets, including nucleic acids, proteins and lipids. DNA is the most important target of UV irradiation. In this study, we evaluated the combined effects of ELF magnetic field exposure on cell survival and DNA damage induced by UV irradiation The ELF magnetic field exposure was conducted with a Helmholtz coil-based exposure system, which was designed to generate a sinusoidal magnetic field at 5 millitesla (mT) and 50/60 Hz. UV irradiation was performed with specially designed apparatus with five UV-Clamps (mainly 253.7 nm) at room temperature. Human embryo lung-derived SV40 virus transformed WI38VA13 subclone 2RA cells were cultured in Eagle's minimum essential medium with non-essential amino acids, lmM sodium pyruvate and 10% fetal bovine serum (FBS). Human xeroderma pigmentosum (valiant type )-derived XP2SA cells were cultured in Dulbecco's modified Eagle's medium with 10% FBS. The cell survival, DNA synthesis and DNA damage were assessed by WST assay, IdU assay and quantification of cyclobutane pyrimidine dimer formation with ELISA method, respectively. WI38VA13 subclone 2RA cells were exposed to magnetic field at 5 mT and 50/60 Hz for 24 hours before/after UV irradiation at 0, 2, 4, 6 and 8 J/m2 • XP2SA cells were analyzed as described for the WI38V A 13 subclone 2RA cells, except that UV irradiation was performed at 0.5, 1, 1.5 and 2 J/m2 for cell survival assay and DNA synthesis assay, and 1, 2, 3 and 4 J/m2 for DNA damage assay. No significant differences were observed in cell survival between ELF magnetic field and sham exposures. Similarly, DNA damages induced by UV irradiation were not changed significantly by ELF magnetic field exposure. Our results suggest that ELF magnetic field exposure at 5 mT does not affect cell survival and DNA damage induced by UV irradiation.}, added-at = {2021-02-12T13:05:48.000+0100}, author = {Mizuno, K and Narita, E and Yamada, M and Shinohara, N and Miyakoshi, J}, biburl = {https://www.bibsonomy.org/bibtex/2a47ec464e2c094e69a9f0b8843f56b42/ceps}, interhash = {37e6a8b78a980fbb89ad3e7b6829524b}, intrahash = {a47ec464e2c094e69a9f0b8843f56b42}, keywords = {DNA ELISA UV-C WST assay cell dimer method pigmentsum pyrimidine survival synthesis type variant xeroderma}, timestamp = {2023-12-20T14:33:19.000+0100}, title = {The combined effects of ELF magnetic field exposure and ultraviolet irradiation}, year = 2013 }