%0 Journal Article %1 Berthoud2009 %A Berthoud, Tamara K. %A Dunachie, Susanna J. %A Todryk, Stephen %A Hill, Adrian V S. %A Fletcher, Helen A. %D 2009 %J J Immunol Methods %K vaccine malaria assay %N 1 %P 33--41 %R 10.1016/j.jim.2008.09.021 %T MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines. %U http://dx.doi.org/10.1016/j.jim.2008.09.021 %V 340 %X For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.

@article{Berthoud2009, abstract = {For many years the IFN-gamma ex vivo ELISPOT has been a major assay for assessing human T-cell responses generated by malaria vaccines. The ELISPOT assay is a sensitive assay, but an imperfect correlate of protection against malaria. Monokine induced by gamma (MIG), or CXCL9, is a chemokine induced by IFN-gamma and has the potential to provide amplification of the IFN-gamma signal. MIG secretion could provide a measure of bio-active IFN-gamma and a functional IFN-gamma signalling pathway. We report that detecting MIG by flow cytometry and by RT-PCR can be more sensitive than the detection of IFN-gamma using these methods. We also find that there is little inter-individual variability in MIG secretion when detected by flow cytometry and that the MIG assay may be used to estimate the amount of bio-active IFN-gamma present. Measurement of MIG alongside IFN-gamma may provide a fuller picture of Th1 type responses post-vaccination.}, added-at = {2012-12-19T02:26:46.000+0100}, author = {Berthoud, Tamara K. and Dunachie, Susanna J. and Todryk, Stephen and Hill, Adrian V S. and Fletcher, Helen A.}, biburl = {https://www.bibsonomy.org/bibtex/21d83336c3cc43d1de3b30dd1a75653b0/aorchid}, doi = {10.1016/j.jim.2008.09.021}, file = {:Vaccine/JImmunolMethods.340.33.pdf:PDF}, groups = {public}, institution = {University of Oxford, Centre for Clinical Vaccinology and Tropical Medicine, Churchill Hospital, Oxford, OX3 7LJ, UK.}, interhash = {3fa2b7d9bd136b393d12c474710cbfd7}, intrahash = {1d83336c3cc43d1de3b30dd1a75653b0}, journal = {J Immunol Methods}, keywords = {vaccine malaria assay}, language = {eng}, medline-pst = {ppublish}, month = Jan, number = 1, pages = {33--41}, pii = {S0022-1759(08)00304-9}, pmid = {18952093}, timestamp = {2012-12-19T02:26:46.000+0100}, title = {MIG (CXCL9) is a more sensitive measure than IFN-gamma of vaccine induced T-cell responses in volunteers receiving investigated malaria vaccines.}, url = {http://dx.doi.org/10.1016/j.jim.2008.09.021}, username = {aorchid}, volume = 340, year = 2009 }