%0 Journal Article %1 raymond_detection_2010 %A Raymond, Philippe %A Gendron, Louis %A Khalf, Moustafa %A Paul, Sylvianne %A Dibley, Kim L %A Bhat, Somanath %A Xie, Vicki R D %A Partis, Lina %A Moreau, Marie-Eve %A Dollard, Cheryl %A Coté, Marie-José %A Laberge, Serge %A Emslie, Kerry R %D 2010 %J Analytical and Bioanalytical Chemistry %K Base_Sequence Genetic_Engineering Genetically_Modified Molecular_Sequence_Data Plant Plants Polymerase_Chain_Reaction Sequence_Alignment Soybeans Zea_mays {DNA} {DNA}_Fingerprinting %N 6 %P 2091--2102 %R 10.1007/s00216-009-3295-6 %T Detection and identification of multiple genetically modified events using DNA insert fingerprinting %U http://www.ncbi.nlm.nih.gov/pubmed/19943159 %V 396 %X Current screening and event-specific polymerase chain reaction (PCR) assays for the detection and identification of genetically modified organisms (GMOs) in samples of unknown composition or for the detection of non-regulated GMOs have limitations, and alternative approaches are required. A transgenic DNA fingerprinting methodology using restriction enzyme digestion, adaptor ligation, and nested PCR was developed where individual GMOs are distinguished by the characteristic fingerprint pattern of the fragments generated. The inter-laboratory reproducibility of the amplified fragment sizes using different capillary electrophoresis platforms was compared, and reproducible patterns were obtained with an average difference in fragment size of 2.4 bp. DNA insert fingerprints for 12 different maize events, including two maize hybrids and one soy event, were generated that reflected the composition of the transgenic DNA constructs. Once produced, the fingerprint profiles were added to a database which can be readily exchanged and shared between laboratories. This approach should facilitate the process of GMO identification and characterization.

@article{raymond_detection_2010, abstract = {Current screening and event-specific polymerase chain reaction {(PCR)} assays for the detection and identification of genetically modified organisms {(GMOs)} in samples of unknown composition or for the detection of non-regulated {GMOs} have limitations, and alternative approaches are required. A transgenic {DNA} fingerprinting methodology using restriction enzyme digestion, adaptor ligation, and nested {PCR} was developed where individual {GMOs} are distinguished by the characteristic fingerprint pattern of the fragments generated. The inter-laboratory reproducibility of the amplified fragment sizes using different capillary electrophoresis platforms was compared, and reproducible patterns were obtained with an average difference in fragment size of 2.4 bp. {DNA} insert fingerprints for 12 different maize events, including two maize hybrids and one soy event, were generated that reflected the composition of the transgenic {DNA} constructs. Once produced, the fingerprint profiles were added to a database which can be readily exchanged and shared between laboratories. This approach should facilitate the process of {GMO} identification and characterization.}, added-at = {2011-08-03T21:06:35.000+0200}, author = {Raymond, Philippe and Gendron, Louis and Khalf, Moustafa and Paul, Sylvianne and Dibley, Kim L and Bhat, Somanath and Xie, Vicki R D and Partis, Lina and Moreau, {Marie-Eve} and Dollard, Cheryl and Coté, {Marie-José} and Laberge, Serge and Emslie, Kerry R}, biburl = {https://www.bibsonomy.org/bibtex/2890e3c39fcfcfa4e45f62a7210260715/crc_chus}, doi = {10.1007/s00216-009-3295-6}, interhash = {40718cbc3b81422039349d27609f692c}, intrahash = {890e3c39fcfcfa4e45f62a7210260715}, issn = {1618-2650}, journal = {Analytical and Bioanalytical Chemistry}, keywords = {Base_Sequence Genetic_Engineering Genetically_Modified Molecular_Sequence_Data Plant Plants Polymerase_Chain_Reaction Sequence_Alignment Soybeans Zea_mays {DNA} {DNA}_Fingerprinting}, month = mar, note = {{PMID:} 19943159}, number = 6, pages = {2091--2102}, timestamp = {2011-08-03T21:10:04.000+0200}, title = {Detection and identification of multiple genetically modified events using {DNA} insert fingerprinting}, url = {http://www.ncbi.nlm.nih.gov/pubmed/19943159}, volume = 396, year = 2010 }