%0 Journal Article %1 Li2019 %A Li, Yiming %A Wu, Yu-Le %A Hoess, Philipp %A Mund, Markus %A Ries, Jonas %D 2019 %I The Optical Society %J Biomedical Optics Express %K 3D Aberration aperture correction internal microscopy numerical psf reflection software %N 6 %P 2708 %R 10.1364/boe.10.002708 %T Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization %V 10 %X Three-dimensional single molecule localization microscopy relies on the fitting of the individual molecules with a point spread function (PSF) model. The reconstructed images often show local squeezing or expansion in z. A common cause is depth-induced aberrations in conjunction with an imperfect PSF model calibrated from beads on a coverslip, resulting in a mismatch between measured PSF and real PSF. Here, we developed a strategy for accurate z-localization in which we use the imperfect PSF model for fitting, determine the fitting errors and correct for them in a post-processing step. We present an open-source software tool and a simple experimental calibration procedure that allow retrieving accurate z-positions in any PSF engineering approach or fitting modality, even at large imaging depths.

@article{Li2019, abstract = {Three-dimensional single molecule localization microscopy relies on the fitting of the individual molecules with a point spread function (PSF) model. The reconstructed images often show local squeezing or expansion in z. A common cause is depth-induced aberrations in conjunction with an imperfect PSF model calibrated from beads on a coverslip, resulting in a mismatch between measured PSF and real PSF. Here, we developed a strategy for accurate z-localization in which we use the imperfect PSF model for fitting, determine the fitting errors and correct for them in a post-processing step. We present an open-source software tool and a simple experimental calibration procedure that allow retrieving accurate z-positions in any PSF engineering approach or fitting modality, even at large imaging depths.}, added-at = {2020-03-23T21:12:34.000+0100}, author = {Li, Yiming and Wu, Yu-Le and Hoess, Philipp and Mund, Markus and Ries, Jonas}, biburl = {https://www.bibsonomy.org/bibtex/2eb6d8a0dbd3e846305e031ff033e895b/kfriedl}, doi = {10.1364/boe.10.002708}, file = {:C$\backslash$:/Users/Karoline/AppData/Local/Mendeley Ltd./Mendeley Desktop/Downloaded/Li et al. - 2019 - Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization.pdf:pdf}, interhash = {46dfb22c0225a8ab61dbe9ad42e6bfd2}, intrahash = {eb6d8a0dbd3e846305e031ff033e895b}, issn = {2156-7085}, journal = {Biomedical Optics Express}, keywords = {3D Aberration aperture correction internal microscopy numerical psf reflection software}, month = jun, number = 6, pages = 2708, publisher = {The Optical Society}, timestamp = {2020-03-23T22:27:15.000+0100}, title = {{Depth-dependent PSF calibration and aberration correction for 3D single-molecule localization}}, volume = 10, year = 2019 }