%0 Journal Article %1 citeulike:489616 %A Vudac, N. %A Mezdour, H. %A Parra, H. J. %A Luc, G. %A C., J. %A Fruchart, %C Service de Recherche sur les Lipoprotéines et l'Athérosclérose, Institut Pasteur de Lille, France. %D 1989 %J Journal of Lipid Research %K apoa elisa apob %N 9 %P 1437--1443 %T A selective bi-site immunoenzymatic procedure for human Lpa lipoprotein quantification using monoclonal antibodies against apoa and apoB. %U http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2532239 %V 30 %X A selective bi-site ELISA assay procedure for quantification of Lpa lipoprotein in human plasma based on linkage of apoa to apoB is described. The lipoproteins referred to as apoa:B were captured by a mixture of two anti-apoa monoclonal antibodies (K07, K09) and were revealed by a mixture of six anti-apoB monoclonal antibodies coupled to peroxidase. Since apoa and plasminogen have striking similarities in protein structure, the selective binding of Lpa:B in our assay depended upon the marked difference in affinity of the K07 and K09 mixture for Lpa:B (Kd = 0.32 x 10(-10) M) versus plasminogen (Kd = 0.47 x 10(-7)M). The high sensitivity (the Lpa:B working range 0.06-0.40 micrograms/ml) and the use of anti-apoB as antibody tracer added to the selectivity of the assay. The expression of K07 and K09 epitopes determined by competitive inhibition method and the reactivity of Lpa:B particles measured by bi-site ELISA were similar on individual lipoproteins, independent to their plasma levels. The assay is precise, and intra- and interassay coefficients of variation were 4.7% and 9.6%, respectively. It yields quantitative Lpa:B values that correlate highly with Lpa levels obtained by electroimmunoassay with polyclonal antibody (r = 0.73) or with Lpa levels measured by the other bi-site ELISA using only K07 and K09 antibodies (r = 0.96). However, upon analyzing each individual plasma with an arbitrary Lpa-cut off of 15 mg/dl, evidence of the qualitative aspect of the lipoprotein was obtained. The group with Lpa less than 15 mg/dl had higher frequency of subjects (65%) with the ratio Lpa/Lpa:B above 1.5.(ABSTRACT TRUNCATED AT 250 WORDS)

@article{citeulike:489616, abstract = {A selective bi-site ELISA assay procedure for quantification of Lp[a] lipoprotein in human plasma based on linkage of apo[a] to apoB is described. The lipoproteins referred to as apo[a]:B were captured by a mixture of two anti-apo[a] monoclonal antibodies (K07, K09) and were revealed by a mixture of six anti-apoB monoclonal antibodies coupled to peroxidase. Since apo[a] and plasminogen have striking similarities in protein structure, the selective binding of Lp[a]:B in our assay depended upon the marked difference in affinity of the K07 and K09 mixture for Lp[a]:B (Kd = 0.32 x 10(-10) M) versus plasminogen (Kd = 0.47 x 10(-7)M). The high sensitivity (the Lp[a]:B working range 0.06-0.40 micrograms/ml) and the use of anti-apoB as antibody tracer added to the selectivity of the assay. The expression of K07 and K09 epitopes determined by competitive inhibition method and the reactivity of Lp[a]:B particles measured by bi-site ELISA were similar on individual lipoproteins, independent to their plasma levels. The assay is precise, and intra- and interassay coefficients of variation were 4.7% and 9.6%, respectively. It yields quantitative Lp[a]:B values that correlate highly with Lp[a] levels obtained by electroimmunoassay with polyclonal antibody (r = 0.73) or with Lp[a] levels measured by the other bi-site ELISA using only K07 and K09 antibodies (r = 0.96). However, upon analyzing each individual plasma with an arbitrary Lp[a]-cut off of 15 mg/dl, evidence of the qualitative aspect of the lipoprotein was obtained. The group with Lp[a] less than 15 mg/dl had higher frequency of subjects (65%) with the ratio Lp[a]/Lp[a]:B above 1.5.(ABSTRACT TRUNCATED AT 250 WORDS)}, added-at = {2006-07-07T01:10:50.000+0200}, address = {Service de Recherche sur les Lipoprotéines et l'Athérosclérose, Institut Pasteur de Lille, France.}, author = {Vudac, N. and Mezdour, H. and Parra, H. J. and Luc, G. and C., J. and Fruchart}, biburl = {https://www.bibsonomy.org/bibtex/23e764801942dceb48180247c9101daa0/biblio24}, citeulike-article-id = {489616}, interhash = {47fe40c54fcf7c698d7a56151560a2cf}, intrahash = {3e764801942dceb48180247c9101daa0}, issn = {0022-2275}, journal = {Journal of Lipid Research}, keywords = {apoa elisa apob}, month = {September}, number = 9, pages = {1437--1443}, priority = {2}, timestamp = {2006-07-07T01:10:50.000+0200}, title = {A selective bi-site immunoenzymatic procedure for human Lp[a] lipoprotein quantification using monoclonal antibodies against apo[a] and apoB.}, url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=pubmed\&dopt=Abstract\&list_uids=2532239}, volume = 30, year = 1989 }