%0 Journal Article %1 claus_differential_2000 %A Claus, H %A Friedrich, A %A Frosch, M %A Vogel, U %D 2000 %J Journal of Bacteriology %K Bacterial, Cells, Clone Deoxyribonucleases, Elements, Enzymes, Genes, Genetics, Insertional, Methylases, Microbial, Modification Mutagenesis, Neisseria Recombinant, Transposable Type meningitidis {DNA-Cytosine} {DNA} {DNA}, {II} {Restriction-Modification} {Site-Specific}, %N 5 %P 1296--1303 %T Differential distribution of novel restriction-modification systems in clonal lineages of Neisseria meningitidis %U http://www.ncbi.nlm.nih.gov/pubmed/10671450 %V 182 %X Using representational difference analysis, we isolated novel meningococcal restriction-modification (R-M) systems. NmeBI, which is a homologue of the R-M system HgaI of Pasteurella volantium, was present in meningococci of the ET-5 complex and of lineage III. NmeAI was found in serogroup A, ET-37 complex, and cluster A4 meningococci. NmeDI was harbored by meningococci of the ET-37 complex and of cluster A4, but not by serogroup A meningococci. Two of the R-M systems, NmeBI and NmeDI, were located at homologous positions between the phenylalanyl-tRNA synthetase genes pheS and pheT, which appeared to be a preferential target for the insertion of foreign DNA in meningococci. The distribution of the three R-M systems was tested with 103 meningococcal strains comprising 49 sequence types. The vast majority of the strains had either NmeBI, NmeAI, or both NmeAI and NmeDI. Using cocultivation experiments, we could demonstrate that NmeBI, which was present in ET-5 complex meningococci, was responsible for a partial restriction of DNA transfer from meningococci of the ET-37 complex to meningococci of the ET-5 complex.

@article{claus_differential_2000, abstract = {Using representational difference analysis, we isolated novel meningococcal restriction-modification {(R-M)} systems. {NmeBI}, which is a homologue of the {R-M} system {HgaI} of Pasteurella volantium, was present in meningococci of the {ET-5} complex and of lineage {III.} {NmeAI} was found in serogroup A, {ET-37} complex, and cluster A4 meningococci. {NmeDI} was harbored by meningococci of the {ET-37} complex and of cluster A4, but not by serogroup A meningococci. Two of the {R-M} systems, {NmeBI} and {NmeDI}, were located at homologous positions between the {phenylalanyl-tRNA} synthetase genes {pheS} and {pheT}, which appeared to be a preferential target for the insertion of foreign {DNA} in meningococci. The distribution of the three {R-M} systems was tested with 103 meningococcal strains comprising 49 sequence types. The vast majority of the strains had either {NmeBI}, {NmeAI}, or both {NmeAI} and {NmeDI.} Using cocultivation experiments, we could demonstrate that {NmeBI}, which was present in {ET-5} complex meningococci, was responsible for a partial restriction of {DNA} transfer from meningococci of the {ET-37} complex to meningococci of the {ET-5} complex.}, added-at = {2011-06-24T11:21:47.000+0200}, author = {Claus, H and Friedrich, A and Frosch, M and Vogel, U}, biburl = {https://www.bibsonomy.org/bibtex/2e6526e6dff72a807f8fba271f98d669f/ag_vogel}, interhash = {4ad2255c68a441162499b446e16839e9}, intrahash = {e6526e6dff72a807f8fba271f98d669f}, issn = {0021-9193}, journal = {Journal of Bacteriology}, keywords = {Bacterial, Cells, Clone Deoxyribonucleases, Elements, Enzymes, Genes, Genetics, Insertional, Methylases, Microbial, Modification Mutagenesis, Neisseria Recombinant, Transposable Type meningitidis {DNA-Cytosine} {DNA} {DNA}, {II} {Restriction-Modification} {Site-Specific},}, month = mar, note = {{PMID:} 10671450}, number = 5, pages = {1296--1303}, timestamp = {2011-06-24T11:21:53.000+0200}, title = {Differential distribution of novel restriction-modification systems in clonal lineages of Neisseria meningitidis}, url = {http://www.ncbi.nlm.nih.gov/pubmed/10671450}, volume = 182, year = 2000 }