%0 Journal Article %1 sandstraem_serology_1980 %A Sandström, E %A Danielsson, D %D 1980 %J Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology %K Acid, Agglutination Antibodies, Antigens, Bacterial, Carbohydrates, Epitopes, Neisseria Periodic Pronase, Serotyping Tests, gonorrhoeae, %N 1 %P 27--38 %T Serology of Neisseria gonorrhoeae. Classification by co-agglutination %U http://www.ncbi.nlm.nih.gov/pubmed/6154404 %V 88 %X The co-agglutination (COA) method has been adapted for serological classification of Neisseria gonorrhoeae. COA reagents were prepared with selectively absorbed rabbit hyperimmune antibodies against gonoccal (GC) major outer membrane protein (MOMP) serotype strains. Using these reagents, the 16 MOMP reference strains could be referred to at least three antigen classes, tentatively named W, J and M. The GC antigens of class W were divided into three groups I, II and III, and they were in part sensitive to pronase. The antigens of class J reflected strain specific or serotype reactions, some sensitive and others resistant to proteolytic enzymes. The antigens of class M were sensitive to periodate and resistant to pronase. Strains used in serological studies by other authors were tested. The properties of class W correlated well with those of the so-called micro-immunofluorescence and immunotype systems, and class M with those of the so-called endotoxin and acid polysaccharide systems. Strains from three different laboratories could all be grouped by class W and M reagents. Identical strains obtained independently from different laboratories gave very similar reaction patterns with the reagents available. Repeated GC-isolates from patients infected with beta-lactamase producing strains showed stable reactions with class W and J reagents, while there was a time-related variation of the class M pattern. We have found that the COA method is rapid, easy and reproducible in the serological classification of Neisseria gonorrhoeae and all the 117 GC-strains tested could be classified.

@article{sandstraem_serology_1980, abstract = {The co-agglutination {(COA)} method has been adapted for serological classification of Neisseria gonorrhoeae. {COA} reagents were prepared with selectively absorbed rabbit hyperimmune antibodies against gonoccal {(GC)} major outer membrane protein {(MOMP)} serotype strains. Using these reagents, the 16 {MOMP} reference strains could be referred to at least three antigen classes, tentatively named W, J and M. The {GC} antigens of class W were divided into three groups I, {II} and {III,} and they were in part sensitive to pronase. The antigens of class J reflected strain specific or serotype reactions, some sensitive and others resistant to proteolytic enzymes. The antigens of class M were sensitive to periodate and resistant to pronase. Strains used in serological studies by other authors were tested. The properties of class W correlated well with those of the so-called micro-immunofluorescence and immunotype systems, and class M with those of the so-called endotoxin and acid polysaccharide systems. Strains from three different laboratories could all be grouped by class W and M reagents. Identical strains obtained independently from different laboratories gave very similar reaction patterns with the reagents available. Repeated {GC-isolates} from patients infected with beta-lactamase producing strains showed stable reactions with class W and J reagents, while there was a time-related variation of the class M pattern. We have found that the {COA} method is rapid, easy and reproducible in the serological classification of Neisseria gonorrhoeae and all the 117 {GC-strains} tested could be classified.}, added-at = {2011-03-11T10:05:34.000+0100}, author = {Sandström, E and Danielsson, D}, biburl = {https://www.bibsonomy.org/bibtex/211f0f285ad3898326828aeeb6d9c2d0a/jelias}, interhash = {5124ed62e6305c64184c0e4244429f32}, intrahash = {11f0f285ad3898326828aeeb6d9c2d0a}, issn = {0105-0656}, journal = {Acta Pathologica Et Microbiologica Scandinavica. Section B, Microbiology}, keywords = {Acid, Agglutination Antibodies, Antigens, Bacterial, Carbohydrates, Epitopes, Neisseria Periodic Pronase, Serotyping Tests, gonorrhoeae,}, month = feb, note = {{PMID:} 6154404}, number = 1, pages = {27--38}, timestamp = {2011-03-11T10:05:55.000+0100}, title = {Serology of Neisseria gonorrhoeae. Classification by co-agglutination}, url = {http://www.ncbi.nlm.nih.gov/pubmed/6154404}, volume = 88, year = 1980 }