%0 Journal Article %1 Bhattacharyya2006Ste5 %A Bhattacharyya, Roby P. %A Reményi, Attila %A Good, Matthew C. %A Bashor, Caleb J. %A Falick, Arnold M. %A Lim, Wendell A. %D 2006 %I American Association for the Advancement of Science %J Science %K cell-cycle cell-fate inbi signalling %N 5762 %P 822--826 %R 10.1126/science.1120941 %T The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway %U http://dx.doi.org/10.1126/science.1120941 %V 311 %X Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active—it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.

@article{Bhattacharyya2006Ste5, abstract = {Scaffold proteins organize signaling proteins into pathways and are often viewed as passive assembly platforms. We found that the Ste5 scaffold has a more active role in the yeast mating pathway: A fragment of Ste5 allosterically activated autophosphorylation of the mitogen-activated protein kinase Fus3. The resulting form of Fus3 is partially active—it is phosphorylated on only one of two key residues in the activation loop. Unexpectedly, at a systems level, autoactivated Fus3 appears to have a negative regulatory role, promoting Ste5 phosphorylation and a decrease in pathway transcriptional output. Thus, scaffolds not only direct basic pathway connectivity but can precisely tune quantitative pathway input-output properties.}, added-at = {2018-12-02T16:09:07.000+0100}, author = {Bhattacharyya, Roby P. and Rem\'{e}nyi, Attila and Good, Matthew C. and Bashor, Caleb J. and Falick, Arnold M. and Lim, Wendell A.}, biburl = {https://www.bibsonomy.org/bibtex/295f983298f3519676212d44b9eee3f49/karthikraman}, citeulike-article-id = {501386}, citeulike-linkout-0 = {http://dx.doi.org/10.1126/science.1120941}, citeulike-linkout-1 = {http://www.sciencemag.org/content/311/5762/822.abstract}, citeulike-linkout-2 = {http://www.sciencemag.org/content/311/5762/822.full.pdf}, citeulike-linkout-3 = {http://www.sciencemag.org/cgi/content/abstract/311/5762/822}, citeulike-linkout-4 = {http://view.ncbi.nlm.nih.gov/pubmed/16424299}, citeulike-linkout-5 = {http://www.hubmed.org/display.cgi?uids=16424299}, day = 10, doi = {10.1126/science.1120941}, interhash = {586126f553b1749ebefbaf53952e1497}, intrahash = {95f983298f3519676212d44b9eee3f49}, issn = {1095-9203}, journal = {Science}, keywords = {cell-cycle cell-fate inbi signalling}, month = feb, number = 5762, pages = {822--826}, pmid = {16424299}, posted-at = {2010-05-21 15:20:08}, priority = {2}, publisher = {American Association for the Advancement of Science}, timestamp = {2018-12-02T16:09:07.000+0100}, title = {The Ste5 Scaffold Allosterically Modulates Signaling Output of the Yeast Mating Pathway}, url = {http://dx.doi.org/10.1126/science.1120941}, volume = 311, year = 2006 }